Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture.

By M. Amit, M. Carpenter, M. Inokuma, C. Chiu, Charles P. Harris, M. Waknitz, J. Itskovitz‐Eldor, James A. Thomson. Developmental Biology, 2000

1,564 citations recorded by the source corpus.

Subfields: Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/e1bedc57b76c7de4920214f27be29b76e29c281c

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Citations in the corpus

Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “Similar to mES cells, hES cells maintained proliferative potential for prolonged periods of culture and retained a normal karyotype even in clonal derivatives (4).”
Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, methodology): “With current protocols, hES cells can be maintained on feeder cells in serum-free medium supplemented with bFGF (Amit et al. 2000).”
Production of De Novo Cardiomyocytes: Human Pluripotent Stem Cell Differentiation and Direct Reprogramming (2012, neutral): “Substantial progress has since been made in controlling hPSC culture, through second-generation media containing the commercial product knockout serum replacement (KSR) and FGF2 (Amit et al., 2000).”
Induction of pluripotent stem cells from adult human fibroblasts by defined factors. (2007, background): “hES cells depend on bFGF for self renewal (Amit et al., 2000), whereas mouse ES cells depend on the LIF/Stat3 pathway (Matsuda et al.”
Multilineage Differentiation from Human Embryonic Stem Cell Lines (2001, background): “Under conditions of low cell density, human ES cell lines are more difficult to propagate in serum, with a cloning efficiency of approximately 0.25% [ 16 ].”
Feeder-free growth of undifferentiated human embryonic stem cells (2001, background): “and can be maintained in vitro for over one year (∼250 population doublings), while remaining karyotypically and phenotypically stabl”
A ROCK inhibitor permits survival of dissociated human embryonic stem cells (2007, background): “They undergo massive cell death particularly after complete dissociation, and the cloning efficiency of dissociated hES cells is generally ≤1”
Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. (2001, methodology): “2 (14) were grown on mitotically inactivated (mitomycin C) MEF feeder layer in culture medium as described previously (10).”
Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells (2005, background): “Addition of bFGF (also called FGF2) to medium containing a commercially available serum replacement allows the clonal culture of hESCs on fibroblast”
Human embryonic stem cells express an immunogenic nonhuman sialic acid (2005, background): “To avoid animal serum, certain proprietary serum replacements are in use, but these also contain animal product”
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative (2007, methodology): “Each laboratory was asked, where possible, to culture their cells in a 'Standard' medium, as previously describe”