Feeder-free growth of undifferentiated human embryonic stem cells

By Chunhui Xu, M. Inokuma, J. Denham, Kathaleen Golds, Pratima Kundu, Joseph D. Gold, M. Carpenter. Nature Biotechnology, 2001

1,858 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/3dc136acae95fb2a4bb16a1adfe4490830de7171

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In Biomedical Engineering

1. G. Keller
2. Kenneth R. Boheler
3. A. Wobus
4. Robert Langer
5. Ali Khademhosseini
6. V. Mohanraj
7. Y. Chen

In Molecular Biology

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2. Kenneth R. Boheler
3. A. Wobus
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In Surgery

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2. Robert Langer
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4. V. Mohanraj
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Citations in the corpus

• Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “The cultivation of hES cells on extracellular matrix proteins, such as Matrigel (a complex mixture of ECM proteins isolated from Engelbreth-Holm-Swarm tumor) and laminin with MEF-conditioned media (401), causes hES cells to express high levels of 6- and 1-integrins, which are involved in cell intera”
• Feeder Layer- and Serum-Free Culture of Human Embryonic Stem Cells1 (2004, background): “In recent years, extensive investigation into improving culture systems for hES cells has yielded three main advances: 1) the ability to grow cells under serum-free conditions [1]; 2) the maintenance of the cells in an undifferentiated state on Matrigel matrix with 100% mouse embryonic fibroblast (M”
• Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, background): “…on feeder cells in serum-free medium supplemented with bFGF (Amit et al. 2000). hES cells can also be grown in the absence of feeder cells, if cultured on matrigel- or laminin-coated plates in medium supplemented with mouse embryonic fibroblast conditioned medium (MEF CM) (Xu et al. 2001).”
• Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation (2005, background): “Directed differentiation of oligodendrocytes and their progenitors from hESCs were maintained and amplified as previously described, in the absence of a feeder layer (Xu et al., 2001).”