A ROCK inhibitor permits survival of dissociated human embryonic stem cells
By Kiichi Watanabe, Morio Ueno, Daisuke Kamiya, Ayaka Nishiyama, Michiru Matsumura, T. Wataya, J. Takahashi, Satomi Nishikawa, Shin-ichi Nishikawa, K. Muguruma and 1 more. 2007
2,092 citations recorded by the source corpus.
Subfields: Molecular Biology
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- R. Weinberg
- Jennifer Nichols
- Wolf Reik
- S. Yamanaka
- Juergen A. Knoblich
- Austin G Smith
- Alexander van Oudenaarden
- Madeline A. Lancaster
Citations in the corpus
- Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex (2013, methodology): “S2 A and A′), we formed aggregates by plating 9 × 10(3) dissociated hESCs into each well of V-bottomed 96-well plates (15) and culture them in medium supplemented with the Rho kinase inhibitor (16) (Fig.”
- Slug and Sox9 Cooperatively Determine the Mammary Stem Cell State (2012, methodology): “Thus, we used a ROCK inhibitor to increase organoid-forming efficiency because it has been shown to promote ESC seeding (Watanabe et al., 2007) and organoid formation by intestinal SCs (Sato et al., 2009).”
- Highly efficient reprogramming to pluripotency and directed differentiation of human cells using synthetic modified mRNA (2010, methodology): “Y27632 ROCK inhibitor (Watanabe et al., 2007) was used at 10 mM in recipient plates until the next media change.”
- Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells. (2014, background): “5 embryos and plated them into low-cellbinding plates in the presence of the Rho kinase inhibitor Y27632, which supports cell survival (Watanabe et al., 2007) (Figures 2C and S3B).”
- Organogenesis in a dish: Modeling development and disease using organoid technologies (2014, background): “Specifically, forebrain tissues are generated by plating mouse (52) or human (53) EBs in 2D and examining adherent cells.”
- Self-formation of optic cups and storable stratified neural retina from human ESCs. (2012, methodology): “In addition, to avoid dissociation-induced apoptosis, we added a ROCK inhibitor (Y-27632) to the SFEBq culture of hESCs, as we reported previously (Watanabe et al., 2007; Ohgushi et al., 2010).”