Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha

By Yan Zeng, R. Yi, Bryan R. Cullen. EMBO Journal, 2005

644 citations recorded by the source corpus.

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Citations in the corpus

Metazoan MicroRNAs (2018, background): “When choosing the hairpins that will enter the pathway, the mammalian Microprocessor prefers a stem of 35±1 bp (Fang and Bartel, 2015), a terminal unstructured loop (Zeng et al., 2005), and single-stranded regions flanking the base of the hairpin (Zeng and Cullen, 2005; Han et al., 2006) (Figure 2).”
Molecular mechanisms of RNA interference (2013, unknown): “Curiously, the only reported evidence for ssRNA-specific binding by any microprocessor component implicates the serine/arginine-rich region of Drosha’s N terminus (102), though this probably explains the microprocessor’s preference for a distal ∼10-nt loop on the pri-miRNA substrate rather than addr”
Evolution and Functional Diversification of MIRNA Genes (2011, background): “The double-stranded stem and flanking regions are both important for DGCR8 binding and subsequent Drosha cleavage;11 bp from the junction of singleand double-stranded RNAs (Zeng and Cullen, 2005; Zeng et al., 2005; Han et al., 2006; Kim et al., 2009).”
Specialization and evolution of endogenous small RNA pathways (2007, background): “Mutational analysis of primary miRNA transcripts indicates that the length of the stem and other structural features, such as the terminal loop, determine accurate processin”
Bioinformatic discovery of microRNA precursors from human ESTs and introns (2006, methodology): “According to the report of Zeng [13], we divided the hairpin structure into two parts, the core and the stem extension.”
Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. (2010, background): “One way to explain this observation is that DGCR8/Drosha interacts directly with the loop of primiRNA stem–loops when recognizing its substrates (Zeng et al. 2005), and that the lack of sequentially diced Drosha-dependent miRNA hairpins in animals reflects the limited reach of this complex.”
Modulation of microRNA processing and expression through RNA editing by ADAR deaminases (2006, background): “They are processed sequentially by two members of the RNase III superfamily, Drosha and Dicer, which recognize the foldback hairpin structure of the miRNA precursor”
The neuronal microRNA system (2006, background): “In collaboration with Pasha, a double-stranded RNA-binding domain protein, Drosha selectively cleaves RNA hairpins that have a relatively large terminal loop (usually ∼10 nucleotides), at a position approximately two helical RNA turns into the stem to produce the pre-miRN”
Current prospects for RNA interference-based therapies (2011, background): “Primary microRNAs (pri-miRNAs) are transcribed by RNA polymerases [156] [157] [158] and are trimmed by the microprocessor complex (comprising Drosha and microprocessor complex subunit DCGR8) into ~70 nucleotide precursors, called pre-miRNAs 67, 159, 160 (left side of the figure).”
MicroRNA biogenesis: coordinated cropping and dicing (2005, background): “So how does the Drosha complex recognize its substrates? This question cannot be fully answered yet, but mutagenesis analyses indicate that the tertiary structure of pri-miRNAs is the primary determinant for substrate specificit”
Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. (2006, background): “Zeng et al. previously suggested that the sites of Drosha cleavage may be determined largely by the distance ( 22 nt) from the terminal loop (Zeng et al., 2005).”
The imprinted H19 noncoding RNA is a primary microRNA precursor. (2007, background): “…structure, consisting of an z30-base-pair (bp) imperfect stem, a large terminal loop, and flanking unstructured RNA sequences, which is necessary and sufficient for recognition by the nuclear RNase III enzyme Drosha acting in concert with its cofactor DGCR8 (Han et al. 2004; Zeng et al. 2005).”
microPrimer: the biogenesis and function of microRNA (2005, background): “The efficient processing of pri-miRNA by Drosha requires: a large terminal loop ( 10 nucleotides) in the hairpin; a stem region that is about one helical turn longer than the slightly more than two helical turns of the stem of the resulting pre-miRNA; and 5 and 3 single-stranded RNA extensions at th”
siRNA vs. shRNA: similarities and differences. (2009, background): “Thus, studies on the synthesis and maturation of miRNAs have provided the groundwork for the synthesis of shRNA [86], particularly the miR-30 based shRNAs”