Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex.

By Jinju Han, Yoontae Lee, Kyu-Hyeon Yeom, Jin-Wu Nam, Inha Heo, Je-Keun Rhee, S. Sohn, Yunje Cho, Byoung-Tak Zhang, V. Narry Kim. Cell, 2006

1,672 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8a1393a87f093993329870529dc61ce2bcb4a135

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Citations in the corpus

An optimized microRNA backbone for effective single-copy RNAi. (2013, background): “Because conserved sequence elements in primiRNAs can impact miRNA biogenesis (Feng et al., 2011; Han et al., 2006), we examined whether any such elements have been altered in the experimental miR-30 backbone.”
Aberrant Expression of Oncogenic and Tumor-Suppressive MicroRNAs in Cervical Cancer Is Required for Cancer Cell Growth (2008, background): “Drosha, in complex with DGCR8, cleaves the pri-miRNA at a specified distance (approximately 11 bp) from the stem–single stranded RNA junction in the nucleus to give a pre-miRNA of an ,60-nt hairpin with a 2-nt 39 overhang [13,14].”
Illuminating the silence: understanding the structure and function of small RNAs (2007, background): “, was recently shown by Kim and colleagues to specifically recognize and bind pri-mRN”
Molecular mechanisms of RNA interference (2013, positive): “DGCR8 recognizes the pri-miRNA’s junction of stem and single-stranded RNA, which likely helps position Drosha for the endonucleolytic cleavage it performs on the stem ∼11 base pairs (bp) from the junction (31).”
RNAi therapeutics: principles, prospects and challenges. (2007, background): “Kim and colleagues suggest that the DGCR8 component of the Microprocessor recognizes the junction of the single stranded and double stranded regions in pri-miRNA transcripts, and cuts about one helical turn into the stem loop structure [41].”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “The most current processing model suggest that DGCR8 recognizes the primiRNA at the ssRNA-dsRNA junction and directs Drosha to a specific cleavage site ~11 base pairs (bp) from the junction on the stem where Drosha cuts to liberate a ~ 60-70 bp miRNA hairpin precursor (pre-miRNA) [78, 80, 81, 83, 86”
MicroRNAs: Processing, Maturation, Target Recognition and Regulatory Functions. (2011, background): “elegans, the DGCR8 homolog is known as Pasha (43, 44).”
Processing of intronic microRNAs (2007, background): “This mutation may instead prevent the catalytic reaction (Han et al, 2006).”
RNA-based Therapeutics- Current Progress and Future Prospects (2012, background): “Also, aptamers can be combined with other types of therapeutic agents to serve as a delivery vehicle for siRNAs (McNamara et al., 2006; Neff et al., 2011; Pastor et al., 2010), enzymes (Chen et al., 2008), and anticancer drugs (Hicke et al., 2006; Hwang et al., 2010).”