Molecular mechanisms of RNA interference
By Ross C. Wilson, Jennifer A. Doudna. Annual Review of Biophysics, 2013
1,018 citations recorded by the source corpus.
Small RNA molecules regulate eukaryotic gene expression during development and in response to stresses including viral infection. Specialized ribonucleases and RNA binding proteins govern the production and action of small regulatory RNAs. After initial processing in the nucleus by Drosha, pre-miRNAs are transported to the cytoplasm, where Dicer cleavage generates mature microRNAs (miRNAs) and short interfering RNAs (siRNAs). These double-stranded products assemble with Argonaute proteins such that one strand is preferentially selected and used to guide sequence-specific silencing of complementary target mRNAs by endonucleolytic cleavage or translational repression. Molecular structures of Dicer and Argonaute proteins, and RNA-bound complexes, have offered exciting insight into the mechanisms operating at the heart of RNA silencing pathways.
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/04b220cb4272f9c7389ee78950fa0bf6a72ff81b
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Citations in the corpus
- Loss-of-function approaches in comparative physiology: is there a future for knockdown experiments in the era of genome editing? (2019, background): “The antisense siRNA strand guides RISC to the target mRNA for degradation or translational repression (Fig. 1A; Hannon, 2002; Novina and Sharp, 2004; Carthew and Sontheimer, 2009; Rao et al., 2009; Wilson and Doudna, 2013; Housden et al., 2017).”
- The Functions of MicroRNAs: mRNA Decay and Translational Repression. (2015, background): “Theminimal RISC is composed of a small RNA and Argonaute protein (Ago) [9].”