Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP

By Haidi zhang, F. Kolb, V. Brondani, E. Billy, W. Filipowicz. EMBO Journal, 2002

685 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/89438957ec009b211492f8a96621cda9ec73eca2

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Citations in the corpus

Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs. (2009, background): “Dicer activity is Mg dependent (Zhang et al. 2002), thus incubating cell extract with varying amounts of MgCl2 may give evidence of Dicer-dependent processing of tRNA.”
The small RNA profile during Drosophila melanogaster development. (2003, background): “…Dicer (Bernstein et al., 2001;and Thomas Tuschl2,* Elbashir et al., 2001b; Grishok et al., 2001; Hutvágner et1Department of Animal Molecular Genetics al., 2001; Ketting et al., 2001; Park et al., 2002; ProvostInstitute of Molecular Genetics et al., 2002; Reinhart et al., 2002; Zhang et al., 2002).”
The widespread regulation of microRNA biogenesis, function and decay (2010, background): “There is also evidence to suggest that proteolysis may play a role in modulating Dicer activit”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “The cytosolic component of miRNA maturation revolves around the RNase III endonuclease Dicer, which has been found in all eukaryotes examined to date except budding yeast [107].”
Single processing center models for human Dicer and bacterial RNase III. (2004, methodology): “Dicer functions in RNA interference and was performed as described (Zhang et al., 2002), and incubations in synthesis of small RNA involved in developmental timing in C.”
siRNA vs. shRNA: similarities and differences. (2009, background): “the long primary transcripts into individual shRNAs with a 2 nt 3′ overhang [89].”
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, background): “Similarly, in humans, while Dicer alone can process dsRNAs and pre-miRNAs efficiently (Zhang et al., 2002, 2004, Provost et al., 2002, Chendrimada et al., 2005), the recruitment of the siRNA/miRNAs to Ago2 requires TRBP (Chendrimada et al., 2005).”
Molecular mechanisms of RNA interference (2013, neutral): “Unlike for Drosophila Dicer-2, RNA processing for human Dicer is ATP independent; thus, the helicase domain is unlikely to be involved in translocation of long dsRNA (103).”
The Drosha-DGCR8 complex in primary microRNA processing. (2004, background): “…(Hammond et al. 2001; Ishizuka et al. 2002; Tabara et al. 2002; Liu et al. 2003), these interacting proteins do not seem to be required for the cleavage reaction itself because purified human Dicer and Drosophila Dicer-2 can catalyze the cleavage reaction (Zhang et al. 2002, 2004; Liu et al. 2003).”
Genetic and Functional Diversification of Small RNA Pathways in Plants (2004, background): “…elegans and human, for example, contain only one DICER (Grishok et al. 2001; Ketting et al. 2001; Knight and Bass 2001; Provost et al. 2002; Zhang et al. 2002), even though both PLoS Biology | http://biology.plosjournals.org May 2004 | Volume 2 | Issue 5 | Page 0648 possess miRNA and siRNA…”
A three-dimensional view of the molecular machinery of RNA interference (2009, background): “Although some invertebrate Dicer proteins (such as Drosophila melanogaster DCR-2) seem to require ATP for processive dicing of long dsRNAs (that is, multiple rounds of cleavage without dissociation from the dsRNA substrate), mammalian Dicer proteins seem to be ATP independen”
Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha (2005, methodology): “Dicer recognizes the 30 2 nt overhang of pre-miRNAs and then cuts B22 nt away to produce the miRNA:miRNA* duplex (Zhang et al, 2002, 2004). miRNAs are then selected over miRNAs* by RISC according to the 50 end base-pairing rule (Khvorova et al, 2003; Schwarz et al, 2003).”
RNA silencing: Methods and protocols (2006, background): “Interestingly, the cleavage can be suppressed by the viral suppressor HC-Pro, leading to elevated levels of SCL6-III and SCL6-IV mRNAs (24).”
siRNA function in RNAi: a chemical modification analysis. (2003, background): “This processing event is catalyzed by Dicer, a highly conserved, dsRNA-specific endonuclease that is a member of the RNase III family (Hammond et al. 2000; Zamore et al. 2000; Bernstein et al. 2001; Hamilton et al. 2002; Provost et al. 2002; Zhang et al. 2002).”