siRNA function in RNAi: a chemical modification analysis.

By Y. Chiu, T. Rana. RNA: A publication of the RNA Society, 2003

865 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/90d205168e8b9334e6fc72349de085cce46e7e7a

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Citations in the corpus

Unlocking the potential of the human genome with RNA interference (2004, background): “Several groups have developed backbone modifications to synthetic siRNAs that provide them with resistance to serum nucleases and should therefore increase the half-life of circulating siRNAs in animal model”
RNAi therapeutics: principles, prospects and challenges. (2007, background): “Importantly, selective modifications of the backbone does not seem to reduce RNAi efficiency [24].”
Development of microRNA therapeutics is coming of age (2014, neutral): “F) modification helps to protect against exonucleases, hence making the guide strand more stable, while it does not interfere with RISC loading (Chiu & Rana, 2003).”
MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5′ region (2004, background): “antisense strand, was sufficient to allow efficient RNAi ( Chiu and Rana, 2002, 2003 ).”
Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing. (2006, background): “4 live 4/C substitutions on the sense strand, antisense strand, or both strands were inactive in transcript silencing (Elbashir et al. 2001; Amarzguioui et al. 2003; Braasch et al. 2003; Chiu and Rana 2003; Czauderna et al. 2003).”
Illuminating the silence: understanding the structure and function of small RNAs (2007, background): “Therefore, helical geometry has been postulated as the major determinant for RNA being a specific trigger of gene silencin”
Perspective: machines for RNAi. (2005, background): “…between a small RNA and its RNA target helps determine if the small RNA directs target cleavage or represses mRNA translation, because an A-form helix must be formed with the target RNA at the center of an siRNA guide strand for cleavage to occur (Chiu and Rana 2003; Haley and Zamore 2004).”
Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain (2004, background): “No specific interactions are directed to the 2′-OH groups, which line the minor groove of the RNA helix, consistent with RNAi not generally being affected by 2′-OH group modifications within the siRNA duplex segmen”
Structural basis for 5′-end-specific recognition of guide RNA by the A. fulgidus Piwi protein (2005, result): “Nevertheless, our results are consistent with previous observations that the 2′-OH is not required for RNAi-mediated event”