Single processing center models for human Dicer and bacterial RNase III.

By Haidi zhang, F. Kolb, Lukasz Jaskiewicz, Eric Westhof, W. Filipowicz. Cell, 2004

1,060 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/c93a0f4b5e293a0c4f31d4ca2a9e3adc663efcf1

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Citations in the corpus

MicroRNA regulation by RNA-binding proteins and its implications for cancer (2011, background): “A PAZ domain within Dicer binds the pre-miRNA 2-nt 3′ overhang, while the RNA-binding domain of Dicer binds the double-stranded stem and defines the site of cleavage by measuring 22 nt from the 3′ overhan”
RNAi therapeutics: principles, prospects and challenges. (2007, background): “Since shRNA and siRNA resembles miRNA precursors before and after Dicer processing, respectively, all component of the miRNA pathway might be clogged by high doses of ectopic RNA.”
A three-dimensional view of the molecular machinery of RNA interference (2009, background): “The two RNaseIII domains of the dimer associate to form a single processing centre, with each catalytic domain responsible for the hydrolysis of one strand in the duple”
Perspective: machines for RNAi. (2005, background): “…siRNAs, siRNAs can be generated in two orientations relative to their thermodynamics: with their more weakly paired 5 end facing forward with respect to the end of the dsRNA where Dicer is thought to initiate (Elbashir et al. 2001a; Zhang et al. 2002, 2004), or with this 5 end facing backwards.”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “Ago2 then cleaves a single-strand of the pre-miRNA 11-12 nucleotides from the end of the on the 3’arm to generate a nicked hairpin structure referred to as “Ago2-cleaved precursor miRNA” or “ac-pre-miRNA”.”
Metazoan MicroRNAs (2018, background): “However, the ideal substrates of Microprocessor give rise to pre-miRNA hairpins that have a 22-bp stem with a 5′ phosphate and a 2-nt 3′ overhang (Fang and Bartel, 2015), which are ideal substrates of the mammalian Dicer (Zhang et al., 2004; Park et al., 2011; Gu et al., 2012).”
The Drosha-DGCR8 complex in primary microRNA processing. (2004, background): “Although Dicer associates with several other proteins (Argonaute proteins in various organisms, RDE-4 in Caenorhabditis elegans, R2D2 in Drosophila, and dFMR1 in Drosophila) (Hammond et al. 2001; Ishizuka et al. 2002; Tabara et al. 2002; Liu et al. 2003), these interacting proteins do not seem to be”
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, background): “Similarly, in humans, while Dicer alone can process dsRNAs and pre-miRNAs efficiently (Zhang et al., 2002, 2004, Provost et al., 2002, Chendrimada et al., 2005), the recruitment of the siRNA/miRNAs to Ago2 requires TRBP (Chendrimada et al., 2005).”
Aberrant Expression of Oncogenic and Tumor-Suppressive MicroRNAs in Cervical Cancer Is Required for Cancer Cell Growth (2008, background): “Our findings are consistent with other reports obtained from biochemical analyses that Dicer uses a 39 end counting rule and measures a distance of ,22 nt from the 39-terminus to cleave both strands of RNA, with 1- to 2-nt shifts in the preferred cleavage position [18,66].”