MicroRNA maturation: stepwise processing and subcellular localization

By Yoontae Lee, Kipyoung Jeon, Jun-Tae Lee, Sunyoung Kim, V. Narry Kim. EMBO Journal, 2002

2,480 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d666a299731315c81436a900cbc8b54ee8267c7f

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Citations in the corpus

Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. (2004, background): “We find that Dicer-1 zymes (Bernstein et al., 2001; Grishok et al., 2001; Ketand Dicer-2 are required for siRNA-directed mRNA ting et al., 2001; Lee et al., 2002).”
Perspective: machines for RNAi. (2005, background): “pri-miRNAs contain stem-loop structures that harbor the miRNA in the 5 or 3 half of the stem (Fig. 1; Lee et al. 2002).”
Microarray analysis of microRNA expression in the developing mammalian brain (2004, background): “Mature microRNAs are excised from a stem-loop precursor that itself can be transcribed as part of a longer primary RNA (pri-miRNA) [24].”
MicroRNA expression profiling in prostate cancer. (2007, background): “Considering that the pri-miRNAs are transient products in the miRNA biogenesis and presumably are rapidly further processed after their synthesis ( 46 ), the expression status of the pri-miRNA and the mature miRNA is not necessarily the same.”
New microRNAs from mouse and human. (2003, methodology): “One of the features of miRNAs is that they can occur in clusters that are coexpressed (Lagos-Quintana et al. 2001, 2002; Lau et al. 2001; Lee et al. 2002).”
siRNA vs. shRNA: similarities and differences. (2009, background): “It is transported to the cytoplasm by exportin 5, a Ran-GTPdependent mechanism [90,91].”
A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses. (2006, background): “…hairpin structure; these hairpins are recognized by a protein complex containing the nuclear RNase enzyme Drosha and processed into z 70-nt hairpin intermediates (pre-miRNAs) (Lee et al. 2002, 2003; Denli et al. 2004; Gregory et al. 2004; Han et al. 2004; Landthaler et al. 2004; Zeng et al. 2005).”
Common SNP in pre-miR-146a decreases mature miR expression and predisposes to papillary thyroid carcinoma (2008, neutral): “1 resulted from allele-specific differences in nuclear processing we examined the production of pre-miR-146a from the pri-miR in HeLa nuclear extract (23) (Fig.”
The microRNAs of Caenorhabditis elegans. (2003, background): “Additional sequence and structural features important for processing of the primary transcript and the hairpin precursors also remain to be identified (Lee et al. 2002).”
Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs. (2004, background): “The biogenesis of miRNAs involves several processing steps and proceeds in animal cells according to the following scheme (Lee et al. 2002).”
Identification of metastasis‐related microRNAs in hepatocellular carcinoma (2008, neutral): “823 Multinodular No 189 (78) 121 (92) 68 (62) Yes 52 (22) 10 (8) 42 (38) <0.”
MicroRNAs in body fluids—the mix of hormones and biomarkers (2011, neutral): “First, miRNA is transcribed in the nucleus by RNA polymerase II as long and capped precursor primary miRNA (pri-miRNA)”
MicroRNA biogenesis and cancer. (2005, methodology): “Previously, an in vitro assay was established to monitor pri-miRNA processing using in vitro transcribed RNAs corresponding particular to pri-miRNAs ( 13 ).”
Human embryonic stem cells express a unique set of microRNAs. (2004, background): “Because clustered miRNA genes are generally transcribed into polycistronic primary transcripts (pri-miRNAs) (Lee et al., 2002), the primers were chosen to bind outside the boundary of the predicted approximately 70 nt stemloop clusters so that we could detect pri-miRNAs covering the entire cluster.”
Metazoan MicroRNAs (2018, methodology): “In animals, canonical miRNAs are transcribed by RNA Polymerase II (Pol II) as part of much longer RNAs called “pri-miRNAs” (Lee et al., 2002; Cai et al., 2004; Lee et al., 2004) (Figure 1).”