Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies

By George L. Sen, Helen M. Blau. Nature Cell Biology, 2005

696 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/7b9e9b738e006ff178a3cc490670343b808fff79

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells. (2009, background): “Components of miRNPs (including miRNAs and AGO and GW182 proteins) and repressed mRNAs are found enriched in processing bodies (P-bodies, also known as GW-bodies), which are cytoplasmic structures involved in the degradation and storage of translationally repressed mRNAs [12–17].”
Many roads to maturity: microRNA biogenesis pathways and their regulation (2009, background): “P-bodies are sites of accumulation of untranslated mRNAs and of multiple enzymes involved in mRNA turnover and translational repression, including Ago proteins and miRNA”
Animal MicroRNAs confer robustness to gene expression and have a significant impact on 3'UTR evolution. (2005, positive): “Animal miRNAs are only partially complementary to their targets and repress their expression, likely by blocking translation initiation and by recruiting miRNPs to processing bodies where degradation might occur (Bagga et al., 2005; Lim et al., 2005; Liu et al., 2005; Pillai et al., 2005; Rehwinkel ”
P bodies and the control of mRNA translation and degradation. (2007, background): “…2006; Muhlrad and Parker, 2005) Worm (Squirrell et al., 2006) Human (Yang et al., 2006) Fly (Behm-Ansmant et al., 2006) Human (Liu et al., 2005a; Sen and Blau, 2005) GW182/AIN-1 Autoantigen containing multiple GW repeats, miRNA/siRNA silencing Worm (Ding et al., 2005) Human (Eystathioy et al.,…”
Persistent DNA damage signaling triggers senescence-associated inflammatory cytokine secretion (2009, neutral): “Nonetheless, we consistently observed a fraction of cells in which most DCP1A, GW182 and MVBs clearly co-localized, which also supports observations made by other”
A role for the P-body component GW182 in microRNA function (2005, background): “Recently, Argonaute proteins, which are key RNAi effector components, and their target mRNAs were shown to localize to cytoplasmic foci known as P-bodies or GW-bodie”
Control of translation and mRNA degradation by miRNAs and siRNAs. (2006, background): “In tissue culture cells, all four versions of the mammalian Argonaute proteins are concentrated in Pbodies and can coimmunoprecipitate with the decapping enzyme (Jakymiw et al. 2005; Liu et al. 2005b; Pillai et al. 2005; Sen and Blau 2005) Similarly, ALG-1, which is one of the Argonaute family membe”
MicroRNA function: multiple mechanisms for a tiny RNA? (2005, background): “…very fact that miRNA-repressed mRNAs are detectable in the PBs suggests that these are not being actively degraded, as visualization of mRNA undergoing degradation in PBs required the depletion of essential proteins in the decay pathway to stabilize the decay intermediates (Sheth and Parker 2003).”
microPrimer: the biogenesis and function of microRNA (2005, background): “In human cells, the core component of the RISCs, Argonaute proteins, together with mRNAs that are targeted for silencing by miRNAs are concentrated in cytoplasmic foci called Processing bodies (P-bodies) (Liu et al., 2005; Pillai et al., 2005; Sen and Blau, 2005).”
miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting. (2006, background): “However, there is now ample evidence to indicate that miRNA regulation can result in mRNAdegradation(Baggaetal.,2005;Limetal.,2005),possibly by recruiting the mRNA to cytoplasmic sites of mRNA storage and degradation referred to as processing bodies (Liu et al., 2005; Sen and Blau, 2005 ).”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “RISC assembly and activation occurs within the Pbodies, which is supported the co-localization of miRNA duplexes and Ago 1-4 proteins to GW182 [183, 189, 190, 203-206].”
How do microRNAs regulate gene expression? (2008, background): “Another important development has been the discovery that Argonaute proteins, miRNAs, and their target mRNAs accumulate in cytoplasmic foci usually known as P-bodies, or processing bodies, which are detected by confocal microscopy and immunostaining or the use of fusions between candidate P-body pro”
MicroRNAS and their regulatory roles in plants. (2006, background): “This suggests that miRNA binding directs the message to the P body, where it can be sequestered from the translation machinery and destabilized (77, 120).”