Single-stranded antisense siRNAs guide target RNA cleavage in RNAi.

By Javier Martinez, Agnieszka Patkaniowska, H. Urlaub, Reinhard Lührmann, Thomas Tuschl. Cell, 2002

1,530 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/6df5df9e7e62ebd9cff3a0f4e841cd7b75a15b06

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Citations in the corpus

Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. (2005, neutral): “(Nykänen et al., 2001; Martinez et al., 2002).”
The imprinted H19 noncoding RNA is a primary microRNA precursor. (2007, background): “The mature miRNA is then incorporated into the RNA-induced silencing complex (RISC), where it acts as a guide RNA to direct RISC to complementary mRNA species (Hammond et al. 2000; Martinez et al. 2002; Schwarz et al. 2002).”
New microRNAs from mouse and human. (2003, background): “Argonaute proteins that are associated with singlestranded siRNAs in the target RNA-cleaving RNA-induced silencing complex (RISC; Hammond et al. 2001; Martinez et al. 2002) were found to be associated with mature miRNAs (Mourelatos et al. 2002; Schwarz et al. 2002).”
siRNAs: applications in functional genomics and potential as therapeutics (2004, background): “Components of the RNAi machinery specifically recognize the siRNA duplex and incorporate a single siRNA strand into a protein comple”
Analysis of Genes and Genomes (2004, background): “The net result of this process is that the target mRNA is efficiently degraded, either through participation in siRNA production, or though the activation of an RNA induced silencing complex (RISC) that will degrade the mRNA–siRNA duplexes (Martinez et al., 2002).”
RNAi therapeutics: principles, prospects and challenges. (2007, background): “Of note, single stranded small antisense RNAs may also be loaded into RISC and guide target mRNA cleavage, however with a much reduced efficiency [13].”
RNA interference in the clinic: challenges and future directions (2010, background): “The dsRNA fragment binds to a protein complex called RNA-induced silencing complex (RISC), and the passenger strand of dsRNA is cleaved and discarded while the guide strand is directed to the 3′ untranslated region (UTR) of the complementary target mRN”
Perspective: machines for RNAi. (2005, background): “A member of the Argonaute family of proteins lies at the heart of all RNA silencing effector complexes (Tabara et al. 1999, 2002; Hammond et al. 2001; Hutvágner et al. 2001; Catalanotto et al. 2002; Martinez et al. 2002; Mourelatos et al. 2002; Djikeng et al. 2003; Shi et al. 2004).”
bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. (2003, background): “…the abil- sion in the clones was considerably higher than the maximal endogenous level at the DV boundary, indicat-ity of miRNAs to inactivate genes by RNAi (Hutvagner and Zamore, 2002; Martinez et al., 2002; Zeng et al., ing that the miRNA is present throughout the disc, though at varying levels.”
Mechanisms of gene silencing by double-stranded RNA (2004, background): “Every RISC or miRNP contains a member of the Argonaute (Ago) protein family; the Ago protein probably binds directly to the RNA in these complexes, although formal evidence for this is lackin”
Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. (2004, background): “The mature miRNA, which forms one strand of this duplex, is then incorporated into a large protein complex, termed the RNA induced silencing complex (RISC), where it functions to guide RISC to complementary mRNA targets (Hammond et al. 2000; Martinez et al. 2002; Schwarz et al. 2002).”
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, background): “A single strand of the siRNA or miRNA duplex is incorporated into a ribonucleoprotein effector complex, known as the RNA-induced silencing complex (RISC) (Hammond et al., 2000, Martinez et al., 2002).”
Gene silencing in mammals by small interfering RNAs (2002, background): “In fact, the human Argonaute protein, EIF2C, purifies as a component of the RNAi-induced silencing complex (RISC”