siRNAs: applications in functional genomics and potential as therapeutics

By Yair Dorsett, Thomas Tuschl. Nature reviews. Drug discovery, 2004

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Analysis of cell-based RNAi screens (2006, background): “In mammalian cells, long dsRNAs induce an interferon response, and therefore short 21 mer RNA duplexes (small interfering RNAs [siRNAs]) are effective in silencing target mRNAs [4,5].”
Inverse statistical problems: from the inverse Ising problem to data science (2017, background): “An example is gene knockdowns, where the expression of one or more genes is reduced, by introducing small interfering RNA (siRNA) molecules into the cell [67] or by other techniques.”
On the art of identifying effective and specific siRNAs (2006, background): “Consequently, siRNAs have become one of the most valuable reagents to functionally annotate genomes and possess great potential as therapeutic”
Genome-scale RNAi screen for host factors required for HIV replication. (2008, background): “This mechanism can be exploited by transfecting siRNAs designed to target specific genes into a cell and evaluating the effect of knocking down the expression of these genes on a cellular process (Dorsett and Tuschl, 2004; Moffat and Sabatini, 2006).”