Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes.

By Christian B. Matranga, Yukihide Tomari, Chanseok Shin, David P. Bartel, Phillip D. Zamore. Cell, 2005

1,240 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/3681ea937a95c5f008c5a2127e2b683d0e9a8335

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In Molecular Biology

Citations in the corpus

Current prospects for RNA interference-based therapies (2011, background): “One strand is the 'guide' strand and directs silencing, with the other strand -the 'passenger' -being degraded 22, 23 .”
siRNA vs. shRNA: similarities and differences. (2009, background): “endonuclease activity necessary to cleave and release the passenger strand of the double-stranded stem [76,77,79].”
Gene silencing in plants using artificial microRNAs and other small RNAs. (2008, background): “Song, J.J., Smith, S.K., Hannon, G.J. and Joshua-Tor, L. (2004) Crystal structure of Argonaute and its implications for RISC slicer activity.”
Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. (2006, background): “It was suggested that miRNA/miRNA* duplexes might be loaded into AGO1 by a passenger-strand cleavage-assisted mechanism in Arabidopsis (Matranga et al. 2005).”
MicroRNAs in cell proliferation, cell death, and tumorigenesis (2006, background): “Alternatively, RISC containing any Ago protein may remove the passenger strand via a bypass mechanism that does not require cleavage and likely involves duplex unwinding (Gregory et al, 2005; Matranga et al, 2005; Rand et al, 2005).”
Origins and Mechanisms of miRNAs and siRNAs (2009, background): “This may be because of the multiple bulges and mismatches typical of miRNA duplexes, which presumably block cleavage by Ago (Matranga et al., 2005).”
Strategies for silencing human disease using RNA interference (2007, background): “RISC activation is initially thought to involve AGO2-mediated cleavage of the sense or passenger strand of the double-stranded siRN”
Illuminating the silence: understanding the structure and function of small RNAs (2007, background): “During the siRISC-assembly process, the passenger strand is destroyed and the removal of this strand facilitates RISC formatio”
Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes. (2006, background): “The Dicergenerated double-stranded small RNAs are recruited by Argonaute, and then a strand (called the passenger strand) is released from Argonaute and the other strand (called the guide strand) remains associated with Argonaute as a guide to regulate gene expression (Matranga et al. 2005).”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, methodology): “The model proposed by Matranga et al. (2005) suggests that RNA duplexes (siRNA and miRNA) are loaded into Ago2 of RISC, which then cleaves the passenger strand, leaving the guide strand bound to Ago2.”