Gene silencing in mammals by small interfering RNAs

By Michael T. McManus, Phillip A. Sharp. Nature reviews genetics, 2002

1,486 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/023dd1c02cf42262162bb46e2d620e800287468a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. Phillip A. Sharp
2. R. Weinberg
3. Guido Kroemer
4. J. Kurreck
5. S. Gottesman
6. J. Blenis
7. R. Anjum
8. T. Rana

In Molecular Biology

1. David M. Sabatini
2. Guido Kroemer
3. Beth Levine
4. J. Blenis
5. R. Weinberg
6. Phillip A. Sharp
7. R. Anjum
8. T. Rana

Citations in the corpus

• Autophagic cell death: the story of a misnomer (2008, background): “Small interfering (si)RNA-mediated knockdown of one gene usually affects thousands of transcripts, meaning that multiple non-overlapping siRNAs should be used independently from each other and that experiments should ideally be controlled by retransfection of the cells with a cDNA that is not affect”
• The small RNA regulators of Escherichia coli: roles and mechanisms*. (2004, background): “In eukaryotic cells, microRNAs and RNAi parallel in many ways the bacterial small RNA regulators, confirming that this level of regulation is widespread and is as central to creating a working organism as are the protein regulators we are used to thinking about (16, 63).”
• Antisense technologies. Improvement through novel chemical modifications. (2003, background): “This opened up the possibility of constructing vectors expressing the interfering RNA for long-term silencing of gene expression in mammalian cells (summarized in [117,120]).”
• Gene regulation by riboswitches (2004, background): “These interactions cause inactivation of the targeted gene by subsequent nuclease processing or by other non-nucleolytic mechanism”
• siRNA function in RNAi: a chemical modification analysis. (2003, background): “The evolutionarily conserved phenomenon RNA interference (RNAi), the process by which specific mRNAs are targeted for degradation by complementary short-interfering RNAs (siRNAs), has increasingly become a powerful tool for genetic analysis and is likely to become a potent therapeutic approach for g”
• RNA Interference: Biology, Mechanism, and Applications (2003, background): “siRNAs have been shown to inhibit infection by human immunodeficiency virus, poliovirus, and hepatitis C virus in cultured cell lines (152).”
• A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference (2003, background): “RNAi has emerged as a rapid and efficient means to manipulate gene function in mammalian cell”
• The RSK family of kinases: emerging roles in cellular signalling (2008, result): “These findings are supported by studies using RNAi technology and the development of several RSK inhibitor”