A Protein Sensor for siRNA Asymmetry

By Yukihide Tomari, Christian B. Matranga, B. Haley, N. Martínez, Phillip D. Zamore. Science, 2004

650 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/67335ebfa1eeacdecd2367354bac200f30115f9f

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Citations in the corpus

Purified Argonaute2 and an siRNA form recombinant human RISC (2005, background): “This feature has been proposed as an important quality check during RISC assembly, and this function has recently been attributed to recognition of those phosphate groups within the RLC, in part by the asymmetry determining factor, R2D2 (ref”
The role of PACT in the RNA silencing pathway (2006, background): “Dicer-2 and R2D2 were found in holo-RISC and in the RISC- loading complex (RLC) of the Drosophila cell extract (Pham et al, 2004; Tomari et al, 2004a).”
Perspective: machines for RNAi. (2005, background): “As unwinding of siRNA proceeds, the Dcr-2/R2D2 heterodimer, probably in the RLC, is exchanged for the core RISC protein, Ago2 (Tomari et al. 2004b).”
Structural basis for 5′-end-specific recognition of guide RNA by the A. fulgidus Piwi protein (2005, background): “, whereas related studies have established that the 5′ phosphate of the partner strand is sensed in the process of RISC assembly by means of R2D2 (ref”
Normal microRNA Maturation and Germ-Line Stem Cell Maintenance Requires Loquacious, a Double-Stranded RNA-Binding Domain Protein (2005, neutral): “[21,24,25].”
Molecular mechanisms of RNA interference (2013, neutral): “The orientation of dicing does not determine guide strand identity (67, 73), and it has been reported that an RNAi dsRBP can act as an asymmetry sensor (18, 89) and/or as a functional bridge between Dicer and Argonaute (10).”
Small RNA sorting: matchmaking for Argonautes (2010, background): “In this regard, R2D2 has been shown to bind the more stable end of the dsRNA duplex, whereas Dicer 2 is positioned at the less-stable end of the duplex, providing a mechanism for orientated AGO2 loadin”
A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis. (2005, background): “DCR2 is required for the biogenesis of siRNAs (Lee et al. 2004) and participates with R2D2 in loading siRNAs into RISC (Liu et al. 2003; Denli et al. 2004; Pham et al. 2004; Tomari et al. 2004).”
microRNA-directed phasing during trans-acting siRNA biogenesis in plants. (2005, background): “The dsRNA binding protein HYL1 is also necessary for miRNA biogenesis (Han et al., 2004; Liu et al., 2003; Pham et al., 2004; Tomari et al., 2004; Vazquez et al., 2004a).”
Argonaute proteins: key players in RNA silencing (2008, background): “, whereas Dcr-2 and the dsRNA-binding protein R2D2 are responsible for the production of siRNA from long, perfectly paired dsRN”
Genomics of microRNA. (2006, background): “siRISC [75,76]. R2D2, the fly cofactor for Dicer-2, binds to the more stable end of the siRNA duplex [ 77 ], thereby orienting the protein complex on the siRNA duplex.”
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, background): “Although Drosophila Dicer-2 (one of the two Dicer paralogs in this organism) can efficiently process long dsRNAs to siRNAs, the loading of the siRNAs into the RISC requires R2D2 (Liu et al., 2003, Tomari et al., 2004).”