Purified Argonaute2 and an siRNA form recombinant human RISC

By Fabiola V. Rivas, Niraj Tolia, Ji‐Joon Song, J. P. Aragón, Jidong Liu, Gregory J. Hannon, L. Joshua-Tor. Nature Structural &Molecular Biology, 2005

816 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b38bf6e3a0dc812ce0c00025ed2fce85dc3059cd

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Citations in the corpus

microPrimer: the biogenesis and function of microRNA (2005, background): “Notably, a subpopulation of Argonaute proteins do not retain all the amino acids that are crucial for RISC catalytic activity, and thus cannot cleave a target RNA even when the small RNA guide is sufficiently complimentary to the target (Liu et al., 2004; Meister et al., 2004b; Rivas et al., 2005).”
Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. (2005, neutral): “that cleaves a single phosphodiester bond in the target RNA, triggering its destruction (Hammond et al., 2001, Okamura et al., 2004; Meister et al., 2004b; Rand et al., 2004; Liu et al., 2004; Song et al., 2004; Rivas et al., 2005 ).”
Control of translation and mRNA degradation by miRNAs and siRNAs. (2006, background): “In vitrospecific cleavage activity is dependent on siRNA–Ago2 binding and it does not need the presence of any other factor (Rivas et al. 2005).”
Origins and Mechanisms of miRNAs and siRNAs (2009, background): “Although single-stranded siRNAs can load directly into puriﬁed Argonaute proteins (Rivas et al., 2005), the double-stranded siRNAs that are generated by Dicer cannot and rely instead upon siRISC assembly pathways (Figure 2).”
MicroRNA function: multiple mechanisms for a tiny RNA? (2005, background): “…double-stranded RNA-binding domain (dsRBD)containing proteins: Pasha functioning with Drosha, R2D2 with Drosophila Dicer-2 (Kim 2005), Loqs with Drosophila Dicer-1 (Forstemann et al. 2005; Jiang et al. 2005; Saito et al. 2005), and TRBP with human Dicer (Chendrimada et al. 2005; Haase et al. 2005).”
The Structure of Human Argonaute-2 in Complex with miR-20a (2012, neutral): “We previously found that we could add synthetic guide sequences of various lengths to hAgo2 purified from bacteria (Rivas et al., 2005).”
Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide. (2008, methodology): “UV-Crosslinking Assay UV-crosslinking assays were performed essentially as described (Rivas et al., 2005).”
The role of PACT in the RNA silencing pathway (2006, background): “…are capable of interacting directly with small RNAs through their PAZ domains (to the 30 end) and through the PIWI and the middle domains (to the 50 end) (Song et al, 2003, 2004; Yan et al, 2003; Lingel et al, 2004; Ma et al, 2004, 2005; Parker et al, 2005; Rivas et al, 2005; Yuan et al, 2005).”
Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation. (2008, background): “Structural studies show that AGO-siRNA 50 end interactions are important for recognition, stability, and fidelity of an active effector complex (Ma et al., 2005; Rivas et al., 2005).”