Differentiation of Embryonic Stem Cells to Insulin-Secreting Structures Similar to Pancreatic Islets

By N. Lumelsky, O. Blondel, P. Laeng, I. Velasco, R. Ravin, R. McKay. Science, 2001

1,550 citations recorded by the source corpus.

Subfields: Endocrinology, Diabetes and Metabolism, Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/4b7eb23036ffe37dccc1b99f682dd1b5edde9516

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In Molecular Biology

1. G. Keller
2. K. Hochedlinger
3. Austin G Smith
4. R. Jaenisch
5. Kenneth R. Boheler
6. A. Wobus
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In Surgery

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2. G. Keller

Citations in the corpus

• Organogenesis in a dish: Modeling development and disease using organoid technologies (2014, background): “Endoderm Thyroid Embryonic chick thyroid (90), adult rat thyroid (91) Thyroid progenitors from mESCs (92) Functional thyroid organoid from mESCs (70) Lung Embryonic chick lung (93) Lung progenitors from mESCs and hiPSCs (92, 94) Bronchioalveolar structures from mouse adult lung stem cells (71) Pancr”
• Embryonic stem cells (2004, background): “Other endodermal cell types that have been obtained from ES cells include hepatocytes (Chinzei et al. 2002; Kuai et al. 2003; Rambhatla et al. 2003) and pancreatic islet cells (Lumelsky et al. 2001; Kim et al. 2003).”
• Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. (2002, positive): “Because ES cells can be differentiated into many therapeutically relevant tissue types including neurons (Lee et al., 2000), cardiac myocytes (Doevendans et al., 2000), and pancreatic β cells ( Lumelsky et al., 2001 ), the strategy employed here is applicable to a variety of genetic diseases that ca”
• Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “The insulin-positive clusters, however, failed to normalize high blood glucose levels in transplantation experiments (213).”
• Embryo-derived stem cells: of mice and men. (2001, background): “Evidence for in vitro differentiation of mouse ES cells into hepatic and pancreatic cells has now been reported (Hamazaki et al. 2001, Lumelsky et al. 2001).”
• In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion. (2003, background): “Although it has not been reported (34), these cells may — as do embryonic stem cells (16) — have the capacity to differentiate into glucose-competent β cells.”
• Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential (2004, methodology): “Cells were treated with factors known to promote the expression of a β-like cell phenotype in other systems (Movassat et al., 2002; Hunziker and Stein, 2000; Zulewski et al., 2001; Lumelsky et al., 2001).”
• Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease (2002, background): “Modification of the above procedure can differentiate ES cells into insulin-secreting structures similar to pancreatic islet”
• Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, background): “The first, a five-step protocol, described by Lumelsky et al. (2001), is an adaptation of a protocol for neural cell differentiation and involves the transfer of serum-induced EBs to serum-free medium followed by treatment with FGF and factors that promote maturation of endocrine cells.”
• How is pluripotency determined and maintained? (2007, background): “…by the fact that the ectopic expression of the tissue-specific transcription factors merely directs the differentiation of ES cells, and that the expression of many tissue-specific transcription factors, such as Pax6 and Pdx1, are detected in ES cells (Lumelsky et al., 2001; Okada et al., 2004).”
• The role of the transcriptional regulator Ptf1a in converting intestinal to pancreatic progenitors (2002, background): “In the case of the pancreas, considerable effort is being expended towards deriving β-cells or islets from embryonic stem (ES) cell”