Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells.

By Yan Zeng, Eric J. Wagner, Bryan R. Cullen. Molecules and Cells, 2002

879 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/38e9479cd540d90cb44ac6c4a249809cce31b922

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. David P. Bartel
2. V. Narry Kim
3. Phillip D. Zamore
4. Thomas Tuschl
5. Bryan R. Cullen
6. Gregory J. Hannon
7. V. Ambros
8. T. Du

In Molecular Biology

1. David P. Bartel
2. V. Narry Kim
3. Phillip D. Zamore
4. Thomas Tuschl
5. Gregory J. Hannon
6. Bryan R. Cullen
7. Gunter Meister
8. Scott M. Hammond

Citations in the corpus

• Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. (2004, background): “Endogenous miRNAs have previously been shown to act like siRNAs by cleaving reporter mRNAs carrying sequences perfectly complementary to miRNA ( Zeng et al. 2002, 2003; Brennecke et al. 2003; Stark et al. 2003).”
• Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. (2003, background): “…plasmid pBC12/CMV and its derivatives pCMVluc-miR-30(P), pCMV-luc-miR-21(P), pCMV-luc-random (Zeng et al. 2003), pCMV-miR-30, pCMV-miR-21 (Zeng and Cullen 2003), pBC12/ MS2-HA-Ran (Yi et al. 2002), and pSuper (Brummelkamp et al. 2002) have been described. pSuper-miR-30 was derived by insertion of…”
• Processing of primary microRNAs by the Microprocessor complex (2004, background): “or interference with protein synthesis by a mechanism that remains unclea”
• MicroRNA genes are transcribed by RNA polymerase II (2004, background): “Most of the flanking sequences are actually dispensable for miRNA biogenesis itself while only about 25–100 nucleotides surrounding both the ends of the stem–loops are required for pri-miRNA processing (Zeng et al, 2002; Lee et al, 2003; Chen et al, 2004).”
• Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. (2004, methodology): “Primer extension assays were performed as previously described (Zeng et al. 2002), except that 50 µg of total RNA, derived from transfected 293T cells, was used.”
• Gene silencing in plants using artificial microRNAs and other small RNAs. (2008, background): “…endogenous miRNA precursors to generate sRNAs that direct gene silencing in either plants or animals (Alvarez et al., 2006; Niu et al., 2006; Parizotto et al., 2004; Schwab et al., 2006; Zeng et al., 2002; Figure 1). miRNA precursors preferentially produce one sRNA duplex, the miRNA– miRNA* duplex.”
• Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. (2006, background): “Recent breakthroughs in RNAi technology have been made by generating shRNA expression cassettes that can mimic a natural miRNA gene (Dickins et al., 2005; Silva et al., 2005; Zeng et al., 2002).”
• MicroRNA biogenesis: coordinated cropping and dicing (2005, background): “In addition, a fully functional mature miRNA can be generated from a plasmid that contains a pri-miRNA segment under the control of a heterologous pol II promote”
• MicroRNA maturation: stepwise processing and subcellular localization (2002, background): “Two recent reports showed that miR-30a-as (originally named miR-30) is less abundant than miR-30a-s (previously termed miR-97) (LagosQuintana et al., 2002; Zeng et al., 2002).”
• RNA polymerase III transcribes human microRNAs (2006, background): “miRNAs are short (17–25 base pairs (bp)) noncoding RNAs that guide cellular machinery to specific messenger RNA”
• Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation. (2005, background): “…cells aimed at recapitulating the mechanism of miRNA regulation have supported the model that imperfect base-pairing between an w22 nt RNA and target sites results in inhibition of protein synthesis without alteration of steady-state mRNA levels (Doench et al., 2003; Zeng et al., 2002, 2003).”
• A combined computational-experimental approach predicts human microRNA targets. (2004, background): “Many miRNAs and siRNAs function by base pairing complementarity is partial, the stability of the target mRNA in not affected but its translation is repressed (Olsen and Ambros 1999; Seggerson et al. 2002; Zeng et al. 2002; Doench et al. 2003).”