Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing.

By Gunter Meister, Markus Landthaler, Yair Dorsett, Thomas Tuschl. RNA: A publication of the RNA Society, 2004

664 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/08d8c6802083bc9d05aff065e69339a90bccb16c

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Citations in the corpus

Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver (2007, neutral): “Previous studies have shown that endogenous miRNAs are effective in cleaving reporter mRNAs harbouring miRNA recognition sequences with perfect complementarity to the miRNA (41,42).”
Specific MicroRNAs Modulate Embryonic Stem Cell–Derived Neurogenesis (2006, positive): “Downloaded from www.StemCells.com by Ole Isacson on August 27, 2006 Inhibition of miRNAs Recently, 2-O-methyl oligonucleotides were characterized as sequence-specific inhibitors of miRNA function and miRNAdirected RISC activity [17, 27, 28 ].”
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster (2006, background): “As microRNA function can be inhibited with specific 2′- O -methyl oligoribonucleotide”
A pancreatic islet-specific microRNA regulates insulin secretion (2004, methodology): “We used antisense 2′- O -methyl oligoribonucleotides to specifically inhibit miRNA”
MicroRNA sponges: progress and possibilities. (2010, methodology): “There are three general methods for miRNA lossof-function studies: genetic knockouts, antisense oligonucleotide inhibitors (Meister et al. 2004; Krützfeldt et al. 2005; Ørom et al. 2006) and sponges (Ebert et al.”
microRNAs as oncogenes and tumor suppressors. (2007, methodology): “This has been adopted by two independent research groups to sequence-specifically inhibit miRNA-and siRNA-induced RNA silencing (Hutvagner et al., 2004; Meister et al., 2004), and inhibit four miRNAs in vivo by modified antisense RNAs (Krutzfeldt et al., 2005).”
The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development. (2007, positive): “746 GENES & DEVELOPMENT To further define the miR-124 function in neurogenesis, we inhibited miR-124 function with 2 -O-methyl (2 -OMe) antisense miR-124 oligoribonucleotides, which specifically inactivate their target miRNA through irreversible binding (Meister et al. 2004).”
MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells (2007, background): “Presently, loss-of-function phenotypes are induced by means of chemically modified antisense oligonucleotides—2′ O -methyl, locked nucleic acid (LNA) and others—which are presumed to pair with and block mature microRNAs through extensive sequence complementarit”
The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation (2006, methodology): “We carried out a reciprocal experiment wherein we transfected C2C12 myoblasts with 2′- O -methyl antisense inhibitory oligoribonucleotides, which have been shown to inhibit the function of miRNA”