MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms

By Yan Zeng, R. Yi, Bryan R. Cullen. Proceedings of the National Academy of Sciences of the United States of America, 2003

973 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ea0eaafafbb4934254ca465663ac337df55e4470

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Citations in the corpus

siRNAs: applications in functional genomics and potential as therapeutics (2004, background): “If siRNAs are not carefully selected, siRNAs having partial complementarity to an mRNA target can act like endogenous miRNAs and repress translatio”
Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs[OPEN] (2013, background): “Translational inhibition by miRNAs is common in animals, where it is generally associated with limited miRNA/target complementarity (Zeng et al., 2003).”
Perspective: machines for RNAi. (2005, background): “…that repress the expression of target genes. miRNAs differ from siRNAs in their biogenesis, not in their functions (Hutvágner and Zamore 2002; Ambros et al. 2003; Aukerman and Sakai 2003; Chen 2004; Chen et al. 2004; Doench et al. 2003; Saxena et al. 2003; Zeng et al. 2003; Yekta et al. 2004).”
Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. (2003, background): “The pCMV-luc-miR-21(P) indicator plasmid (Zeng et al. 2003) is identical to pCMV-luc-miR-30(P) (Fig.”
Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha (2005, methodology): “pCMV-miR-30a, pCMV-miR-30a(GAG), pCMV-miR-21, pCMV-miR21(GGU), pSuper-miR-30a, pCMV-luc-8xmiR-30a(P), pCMV-luc8xmiR-21(P), and pH1-GFP have been described (Zeng and Cullen, 2003, 2004; Zeng et al, 2003).”
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs (2005, background): “This observation, together with the documented overlap between the potential activities of siRNAs and metazoan miRNA”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “The most current processing model suggest that DGCR8 recognizes the primiRNA at the ssRNA-dsRNA junction and directs Drosha to a specific cleavage site ~11 base pairs (bp) from the junction on the stem where Drosha cuts to liberate a ~ 60-70 bp miRNA hairpin precursor (pre-miRNA) [78, 80, 81, 83, 86”
A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses. (2006, background): “…perfect complementarity, thereby directing cleavage of the target in an analogous fashion to siRNA-mediated cleavage (Hutvagner and Zamore 2002; Zeng et al. 2003; Mansfield et al. 2004; Yekta et al. 2004). miRNAs are initially transcribed as a primary precursor molecule (pri-miRNA) that can be…”
The Microprocessor complex mediates the genesis of microRNAs (2004, background): “The present model for the pathway by which mammalian miRNAs undergo maturation begins with cleavage of the pri-miRNA in the nucleus to release a ∼60–70-nt stem loop intermediate, known as the pre-miRN”
Specificity of microRNA target selection in translational repression. (2004, background): “As we and others have previously demonstrated, an siRNA can translationally repress a target mRNA with imperfectly complementary binding sites in its 3 UTR, and thus the siRNA functions as an miRNA (Doench et al. 2003; Saxena et al. 2003; Zeng et al. 2003).”
Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. (2004, background): “However, these miRNAs can also guide RNA degradation if presented an artificial substrate with a high degree of complementarity (Hutvágner & Zamore 2002; Brennecke and Cohen 2003; Brennecke et al. 2003; Zeng et al. 2003).”
Thermodynamics of RNA-RNA Binding (2006, background): “Since siRNAs can also function as miRNAs (Doench et al., 2003; Zeng et al., 2003), the siRNA might act in a miRNA like fashion binding to this alternative target site and contribute to the remaining translational repression of this construct.”
Molecular mechanisms of translational control (2004, background): “Even though miRNAs and siRNAs arise from different precursors, they share common processing steps (BOX 1) .”