Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment.

By B. Sacchetti, A. Funari, S. Michienzi, S. Di Cesare, S. Piersanti, I. Saggio, E. Tagliafico, S. Ferrari, P. Robey, M. Riminucci and 1 more. Cell, 2007

2,187 citations recorded by the source corpus.

The identity of cells that establish the hematopoietic microenvironment (HME) in human bone marrow (BM), and of clonogenic skeletal progenitors found in BM stroma, has long remained elusive. We show that MCAM/CD146-expressing, subendothelial cells in human BM stroma are capable of transferring, upon transplantation, the HME to heterotopic sites, coincident with the establishment of identical subendothelial cells within a miniature bone organ. Establishment of subendothelial stromal cells in developing heterotopic BM in vivo occurs via specific, dynamic interactions with developing sinusoids. Subendothelial stromal cells residing on the sinusoidal wall are major producers of Angiopoietin-1 (a pivotal molecule of the HSC "niche" involved in vascular remodeling). Our data reveal the functional relationships between establishment of the HME in vivo, establishment of skeletal progenitors in BM sinusoids, and angiogenesis.

Subfields: Cell Biology, Hematology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d645d1e340b015796c8c68ed30f97507c8b0fceb

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

1. S. Morrison
2. H. Augustin
3. A. Caplan
4. K. Alitalo
5. D. Scadden
6. G. Karsenty
7. M. Lutolf
8. G. Koh

In Hematology

1. S. Morrison
2. A. Caplan
3. P. Robey
4. D. Scadden
5. P. Bianco
6. Lei Ding
7. T. Suda
8. W. Fibbe

In Molecular Biology

1. S. Morrison
2. Fanxin Long
3. K. Alitalo
4. D. Scadden
5. Shenghui He
6. Daisuke Nakada
7. Helen M. Blau
8. T. Suda

Citations in the corpus

• Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. (2008, background): “Angiopoietin-1 expression has been attributed to both osteoblasts at the endosteum (Arai et al., 2004) and to perivascular mesenchymal progenitors (Sacchetti et al., 2007).”
• Mesenchymal stromal cells: sensors and switchers of inflammation. (2013, positive): “As part of the BM niche, MSCs support hematopoiesis and restore the differentiated compartment of osteoblasts and adipocytes during tissue growth and turnover (Sacchetti et al., 2007).”
• Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow (2009, background): “Although cultured MSCs are multipotent in vitro, the cells often commit to the osteocyte lineage after local implantation (Sacchetti et al., 2007).”
• Endothelial and perivascular cells maintain haematopoietic stem cells (2012, background): “Others found that perivascular stromal cells secrete high levels of CXCL12 and other factors proposed to regulate HSC maintenanc”
• Leptin Receptor-expressing mesenchymal stromal cells represent the main source of bone formed by adult bone marrow (2014, positive): “Consistent with the conclusion that MSCs secrete factors that promote HSCmaintenance (Méndez-Ferrer et al., 2010; Omatsu et al., 2010; Sacchetti et al., 2007), both Scf-GFP bone marrow cells and Cxcl12-DsRed bone marrow cells were highly enriched for CFU-Fs (Figures 2B and 2C).”
• From estrogen-centric to aging and oxidative stress: a revised perspective of the pathogenesis of osteoporosis. (2010, background): “(312) demonstrated that the pericytes (also known as mural cells) associated with the abluminal surface of vessels and sinusoids of the bone marrow are the MSC progenitors of osteoblasts.”
• Mechanisms of mesenchymal stem/stromal cell function (2016, background): “This is exemplified by their natural roles as reticular cells that support the hematopoietic stem cell niche [26–28, 70, 71] and as vascular pericytes that support endothelial cells [72, 73].”
• Mesenchymal stem cells in health and disease (2008, background): “However, in the bone marrow, stromal cells are a rare and heterogeneous population of cells that contain a mixture of progenitors at different stages of commitment to the mesodermal lineage and only a very small number of multipotential self-renewing stem cells, which have recently been identified”
• Mesenchymal stem cells: revisiting history, concepts, and assays. (2008, background): “BMSCs also localize to sinusoidal walls, and when hematopoietic development is modeled in vivo, they do so prior to the establishment of hematopoiesis (Sacchetti et al., 2007).”
• Mechanisms involved in the therapeutic properties of mesenchymal stem cells. (2009, background): “In vivo, adventitial reticular cells, which are putative MSCs in vivo [42], sustain the hematopoietic stem cell pool through the secretion of stromal-derived factor 1 (SDF-1, aka CXCL12) [85], which suggests that the hematopoietic support observed in vitro mimics some aspects of that observed under ”
• Control of vascular morphogenesis and homeostasis through the angiopoietin–Tie system (2009, background): “Correspondingly, subendothelial stromal cells in the bone marrow that express the mesenchymal marker molecule CD146 (also called melanoma cell-adhesion molecule (MCAM)) have recently been identified as a major source of ANG1 (Ref”
• Mechanisms of stem cell self-renewal. (2009, background): “Angiopoietin-1, a factor proposed to regulate HSC quiescence (Arai et al. 2004), has been suggested to be expressed by osteoblasts at the endosteum (Arai et al. 2004) as well as by perivascular mesenchymal progenitors (Sacchetti et al. 2007).”
• Effects of dexamethasone, ascorbic acid and β-glycerophosphate on the osteogenic differentiation of stem cells in vitro (2013, background): “In this context, it is important to note that the prolonged culture of bone marrow stromal cells (BMSCs) can deplete the SSC pool or lead to over-dilution of SSCs in the heterogeneous BMSC population [1,6].”