Leptin Receptor-expressing mesenchymal stromal cells represent the main source of bone formed by adult bone marrow
By B. Zhou, R. Yue, M. Murphy, James G. Peyer, S. Morrison. Cell Stem Cell, 2014
1,240 citations recorded by the source corpus.
SUMMARY Studies of the identity and physiological function of mesenchymal stromal cells (MSCs) have been hampered by a lack of markers that permit both prospective identification and fate mapping in vivo. We found Leptin Receptor (LepR) is a marker that highly enriches bone marrow MSCs. Approximately 0.3% of bone marrow cells were LepR+, 10% of which were CFU-F, accounting for 94% of bone marrow CFU-F. LepR+ cells formed bone, cartilage, and adipocytes in culture and upon transplantation in vivo. LepR+ cells were Scf-GFP+, Cxcl12-DsRedhigh, and Nestin-GFPlow, markers which also highly enriched CFU-F, but negative for Nestin-CreER and NG2-CreER, markers which included few CFU-F. Fate-mapping showed LepR+ cells arose postnatally and gave rise to most bone and adipocytes formed in adult bone marrow, including bone regenerated after irradiation or fracture. LepR+ cells were quiescent but proliferated after injury. LepR+ cells are the major source of bone and adipocytes in adult bone marrow.
Subfields: Genetics, Hematology, Orthopedics and Sports Medicine
View Paper: https://www.semanticscholar.org/paper/905936b8add11c6a973680aab52536a6f0fd4004
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In Genetics
Citations in the corpus
- Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration (2017, background): “In support of this observation, clonal analysis of PaS or LepR+ cells showed multipotency only in a smaller subset of clones that may have derived from sub-populations expressing CD24 (Morikawa et al., 2009a; Zhou et al., 2014).”
- Gremlin 1 Identifies a Skeletal Stem Cell with Bone, Cartilage, and Reticular Stromal Potential (2015, methodology): “Recently, perisinusoidal mesenchymal cells expressing Lepr were found to include multipotent, colony-forming unit-fibroblasts (CFU-Fs) (Zhou et al., 2014).”
- Identification and Specification of the Mouse Skeletal Stem Cell (2015, neutral): “…consists of eight different cellular subpopulations with distinct skeletogenic properties and encompasses subpopulations that have characteristics of described skeletogenic cell types identified by lineage tracing (Bianco, 2014, Méndez-Ferrer et al., 2010; Park et al., 2012; Zhou et al., 2014).”