Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow

By S. Morikawa, Y. Mabuchi, Y. Kubota, Y. Nagai, Kunimichi Niibe, Emi Hiratsu, Sadafumi Suzuki, Chikako Miyauchi-Hara, Narihito Nagoshi, Takehiko Sunabori and 6 more. Journal of Experimental Medicine, 2009

792 citations recorded by the source corpus.

Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRα+Sca-1+CD45−TER119−) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.

Subfields: Genetics, Hematology, Oncology

View Paper: https://www.semanticscholar.org/paper/a578fdbd15550cf5563e3f6a5740630f19d7f7f9

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. S. Morrison
2. Fanxin Long
3. H. Kronenberg
4. Lei Ding
5. D. Scadden
6. T. Stappenbeck

In Hematology

1. S. Morrison
2. D. Scadden
3. Lei Ding
4. P. Frenette

In Oncology

1. S. Morrison
2. Fanxin Long
3. D. Scadden
4. G. Karsenty

Citations in the corpus

• Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration (2017, methodology): “Previous work has also shown that neural crest-derived, Nestin+ cells are the likely developmental origin of the adult mesenchymal cells described here (Isern et al., 2014; Morikawa et al., 2009b; Nagoshi et al., 2008; Takashima et al., 2007).”
• Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle (2011, background): “Recently, Morikawa and colleagues reported the prospective isolation of multipotent mesenchymal stem cells in murine bone marrow (Morikawa et al., 2009).”
• The elusive nature and function of mesenchymal stem cells (2011, positive): “Furthermore, in mice combined expression of surface cell antigen 1 (SCA1) and platelet-derived growth factor receptor-α (PDGFRα) specifies a subset of non-haematopoietic cells that resides close to arteries and gives rise to osteoblasts, reticular cells and adipocytes in vivo upon transplantation ”
• Building strong bones: molecular regulation of the osteoblast lineage (2011, background): “More recently, numerous molecular markers have been reported to enrich the progenitor population from bone marrow stromal cell”
• Endothelial and perivascular cells maintain haematopoietic stem cells (2012, background): “This indicates that Scf -GFP + cells included mesenchymal stem/stromal cell”
• Endogenous Bone Marrow MSCs Are Dynamic, Fate-Restricted Participants in Bone Maintenance and Regeneration (2012, background): “Mx1-Induced Progenitors Are Clonogenic and Have Multilineage Differentiation Potential In Vitro Others have demonstrated that mesenchymal stem/progenitor cells are enriched in the Sca1+CD90+ or Sca1+CD140a+ bone marrow fractions (Chan et al., 2009; Morikawa et al., 2009).”
• Gremlin 1 Identifies a Skeletal Stem Cell with Bone, Cartilage, and Reticular Stromal Potential (2015, background): “, 2010), which enhances the yield of CFU-Fs (Morikawa et al., 2009).”
• Leptin Receptor-expressing mesenchymal stromal cells represent the main source of bone formed by adult bone marrow (2014, positive): “Consistent with a prior study (Morikawa et al., 2009), 9.8% ± 5.0% of PDGFRa+CD45 Ter119 bone marrow stromal cells, 15.6% ± 3.0% of PDGFRa+Sca-1+CD45 Ter119 bone marrow stromal cells, and 8.3% ± 5.2% of PDGFRa+Sca-1 CD45 Ter119 bone marrow stromal cells formed CFU-F colonies (Figure 2C).”
• Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle (2010, background): “Recently, PDGFRα + cells have been isolated from mouse bone marrow and characterized as a population highly enriched for MSC”