Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells

By R. Pillai, S. Bhattacharyya, C. Artus, Tabea Zoller, Nicolas Cougot, E. Basyuk, E. Bertrand, W. Filipowicz. Science, 2005

1,534 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/9673c51c2b06200717ba14b8eb30812ddbbd136d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

In Molecular Biology

Citations in the corpus

Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation (2018, background): “miRISC has also been found to copurify with polysomes, and the miRISC:mRNA complexes bound to these polysomes were associated with increased levels of translational inhibition and mRNA degradation (83).”
Argonaute proteins: key players in RNA silencing (2008, background): “Furthermore, tethering translation initiation factors on the miRNA-targeted RNA abrogates miRNA function, and certain internal ribosome entry site elements render the RNA resistant to miRNA-mediated repressio”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “Multiple complementary sites with imperfect basepairing create bulges in the RNA duplex inhibit the slicer activity of Ago2 [2, 3, 20, 174, 183, 184].”
Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis Is Mediated by Downregulation of miR398 and Important for Oxidative Stress Tolerance[W] (2006, background): “, 2005), although mRNA cleavage might also occur (Mansfield et al., 2004; Yekta et al., 2004; Bagga et al., 2005; Pillai et al., 2005).”
P bodies and the control of mRNA translation and degradation. (2007, background): “P bodies also contain mRNAs (Teixeira et al., 2005; Pillai et al., 2005; Liu et al., 2005a), and when semipurified P bodies are treated with RNase A, P bodies are disrupted (Teixeira et al., 2005).”
microPrimer: the biogenesis and function of microRNA (2005, background): “(P-bodies are also known as cytoplasmic bodies or GW-bodies.) miRNAs may initially block translational initiation, causing the miRNA-programmed RISC and the target mRNA to be re-localized to the P-body (Pillai et al., 2005).”
The miRNA156/157 recognition element in the 3' UTR of the Arabidopsis SBP box gene SPL3 prevents early flowering by translational inhibition in seedlings. (2007, background): “mals, worms and insects (Humphreys et al., 2005; Olsen and Ambros, 1999; Pillai et al., 2005).”
Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis (2009, background): “Therefore, mRNAs in polysomes are actively translated, although a minor subpopulation of these may be stalled in initiation or elongation as a result of miRNA-mediated repression or another mechanism (14,15).”
Regulation of mRNA translation and stability by microRNAs. (2010, background): “Analysis of mRNA levels failed to detect pronounced degradation of miRNAtargeted mRNAs, demonstrating that translation was indeed inhibited (46, 91).”
Mechanisms of microRNA‐mediated gene regulation (2007, background): “By contrast, two other reports have demonstrated miRNA-mediated inhibition at the level of initiation of protein synthesis [ 19 ,20] (Figure 2). In one study, an endogenous miRNA was shown to reduce polysome association of a reporter construct with appropriate target sites [19].”
MicroRNAs in development and disease. (2011, background): “A second mode of function employed by miRNAs is inhibition of translation initiation, which requires both the cap (253, 487, 608) and poly(A) tail structures (253).”
Assessing the ceRNA hypothesis with quantitative measurements of miRNA and target abundance. (2014, background): “MicroRNAs (miRNAs) are an abundant class of small, non-coding RNAs that regulate gene expression at the levels of mRNA stability and translation (Pillai et al., 2005; Eulalio et al., 2008; Guo et al., 2010).”
The let-7 microRNA represses cell proliferation pathways in human cells. (2007, background): “, oncogenes or tumor suppressors, to repress protein expression from the target mRNA (10), but miRNAs can also destabilize target mRNAs (11, 12).”
MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer (2008, background): “MicroRNAs (miRNAs) have been discovered as naturally occuring non-coding RNAs, controlling gene expression via specific sites at the 30-UTR of target-mRNAs, causing translational repression or degradation (Berezikov et al., 2005; Pillai et al., 2005; Zamore and Haley, 2005).”