Assessing the ceRNA hypothesis with quantitative measurements of miRNA and target abundance.

By Rémy Denzler, Vikram Agarwal, Joanna Stefano, David P. Bartel, M. Stoffel. Molecules and Cells, 2014

653 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/123a4d755fd4a8d526ad9606cb1983afb76db89a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

In Molecular Biology

Citations in the corpus

Circular RNAs: analysis, expression and potential functions (2016, background): “Still, in order to have an appreciable effect on the concentration of an RBP in the cell without an enrichment of RBP-binding sites, a circRNAwould have to be very highly expressed, making an RBP or miRNA sponge function unlikely for most circRNAs (Denzler et al., 2014), but, of course, rare cases m”
Past, present, and future of circRNAs (2019, critical): “This matter contra-dicts possible sponge theories (Denzler et al , 2014; Jens & Rajewsky, 2015), since the miRNA might be more abundant than the total RNA in which the miRNA can bind and titrate, and there are more miRNA-binding sites available than miRNA molecules.”
The biochemical basis of microRNA targeting efficacy (2018, result): “These results support the inference that the summed contributions of background binding and low-affinity sites to intracellular AGO occupancy are of the same order of magnitude as those of canonical sites, suggesting that an individual AGO-miRNA complex spends about half its time associated with a v”
Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor (2015, background): “Furthermore, according to recent studies, target repression is released only after adding many competing target sites, independently from miRNA levels (52), and, similarly, thousands of binding sites per cell are required to significantly affect binding of well-expressed miRNAs (51,53).”
Mechanisms and Functions of Long Non-Coding RNAs at Multiple Regulatory Levels (2019, background): “Under normal homeostatic conditions, ceRNA-mediated gene regulation is unlikely to occur [66,67].”
Circular RNAs: diversity of form and function (2014, background): “However, given the low abundance of most circRNAs, this may not substantially affect target molecule levels (Denzler et al. 2014).”