Effects of dietary polyunsaturated fatty acid/vitamin E (PUFA/tocopherol ratio on antioxidant defence mechanisms of juvenile gilthead sea bream (Sparus aurata L., Osteichthyes, Sparidae)

By G. Mourente, E. Dı́az-Salvago, D. Tocher, J. Bell. Fish Physiology & Biochemistry, 2000

83 citations recorded by the source corpus.

Lipid peroxidation, specifically polyunsaturated fatty acid (PUFA) oxidation is highly deleterious, resulting in damage to cellular biomembranes, and may be a principal cause of several diseases in fish including jaundice and nutritional muscular dystrophy. Tissue lipid PUFA content and composition are critical factors in lipid peroxidation, as is the level of endogenous antioxidant molecules such as vitamin E. The primary objective of the present study was the characterization of antioxidant systems in a cultured juvenile marine fish, gilthead sea bream (Sparus aurata) with the underlying aim to understand how to avoid oxidation problems that may cause pathologies and disease and so to enhance growth and quality of early ongrowing stages. Juvenile sea bream were fed diets having either high or low levels of fish oil and supplemented or basal levels of vitamin E with PUFA/vitamin E ratios ranging from 117±12 in the diet with low PUFA supplemented with vitamin E to 745±48 in the diet with high PUFA with no additional vitamin E. None of the diets had serious deleterious effects on growth or survival of the fish, but the different dietary regimes were effective in significantly altering the PUFA/vitamin E ratios in the fish livers with values ranging from 5.7±0.4 in fish fed the diet with low PUFA supplemented with vitamin E to 91.1±13.2 in fish fed the diet with high PUFA with no additional vitamin E. This had effects on the peroxidation status of the fish as indicated by the significantly altered levels of in vivo lipid peroxidation products measured in liver, with fish fed the diet rich in PUFA and low in vitamin E showing significantly higher values of thiobarbituric acid reactive substances (TBARS) and isoprostanes. The isoprostane levels generally followed the same pattern as the TBARS levels supporting its value as an indicator of in vivo oxidative stress in fish, as it is in mammals. However, few significant effects on antioxidant enzyme activities were observed suggesting that more severe conditions may be required to affect these activities such as increasing the PUFA/vitamin E ratio or by increasing peroxidative stress through the feeding of oxidized oils.

Subfields: Aquatic Science, Biochemistry, Health, Toxicology and Mutagenesis

View Paper: https://www.semanticscholar.org/paper/32a3637c462d9af7c5752bc790b36b468c42ee97

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In Aquatic Science

1. D. Tocher
2. J. Bell
3. G. Mourente
4. Y. Olsen
5. G. Turchini
6. Wing Ng
7. Ester Santigosa
8. Marie Vagner

In Biochemistry

1. D. Tocher
2. G. Mourente
3. J. Bell

In Health, Toxicology and Mutagenesis

1. J. Bell
2. D. Tocher

Citations in the corpus

• Effects of dietary vitamin E on antioxidant defence mechanisms of juvenile turbot (Scophthalmus maximus L.), halibut (Hippoglossus hippoglossus L.) and sea bream (Sparus aurata L.) (2002, background): “No clear relationship between PUFA/vitamin E ratios and liver antioxidant enzyme activities were observed in gilthead sea bream fed with diets varying in PUFA/vitamin E ratios (Mourente et al. 2000) or during early development in unfed common dentex (Dentex dentex) larvae (Mourente et al. 1999a).”
• Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream (Sparus aurata L.) fed dietary oxidised oil: attenuation by dietary vitamin E (2002, background): “Our previous study investigated the antioxidant systems in cultured juvenile gilthead sea bream by feeding diets varying widely in PUFA/vitamin E ratio through having either high or low levels of fish oil (n 3 HUFA) combined with the presence or absence of vitamin E (Mourente et al., 2000).”
• Does dietary tocopherol level affect fatty acid metabolism in fish? (2007, background): “Recently, we showed that the level of dietary tocopherol had significant effects on the activities of the enzymes of the liver antioxidant defence system in juvenile marine fish (Mourente et al. 2000; Tocher et al. 2002).”
• Antioxidant Defenses in Fish: Biotic and Abiotic Factors (2005, unknown): “…induction of such defenses in fish fed with high PUFA diets (Stephan et al., 1995; Hamre et al., 1997; Olsen et al., 1999; Lygren et al., 2000; Mourente et al., 2000; Tocher et al., 2002b; Kiron et al., 2004), suggesting that dietary vitamin E above a minimum requirement does not…”