Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses

By K. Rothkamm, M. Löbrich. Proceedings of the National Academy of Sciences of the United States of America, 2003

1,724 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/125c653292a6d8d86c4e59a33b3b67e590337d0c

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In Cancer Research

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In Radiology, Nuclear Medicine and Imaging

Citations in the corpus

Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage (2005, background): “Activation of DNA-PK is required for the efficient repair of radiation-induced DSBs, which can be measured by quantitative analysis of γH2AX foci after irradiatio”
Large effects from small exposures. III. Endocrine mechanisms mediating effects of bisphenol A at levels of human exposure. (2006, background): “Other examples of unique low-dose effects not predicted by high-dose studies are 1) the finding of greater reduction in fertility in mice at low relative to high doses of pesticides (122), 2) increased DNA damage at low relative to high doses of x-rays (123), and 3) stimulation of human prostate can”
DNA damage-induced cell death: from specific DNA lesions to the DNA damage response and apoptosis. (2013, background): “In addition to the base lesions, one Gy also induces the formation of 1000 DNA single-strand breaks (SSBs) [58] and 35 DSBs [59] per cell.”
ATM and DNA-PK Function Redundantly to Phosphorylate H2AX after Exposure to Ionizing Radiation (2004, neutral): “Further analysis was as described previously (32).”
Advances in Giant Magnetoresistance Biosensors With Magnetic Nanoparticle Tags: Review and Outlook (2008, background): “Other examples of well known biomarkers include cardiac troponin T (cTnT, a regulatory protein found in cardiac muscle) for myocardial infarction (heart attack) [52], phosphorylation of a special form of histone protein ( -H2AX) in cells for ionizing radiation exposure [ 53 ], and mutation of BRCA1 ”
γ-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin (2008, background): “that the DNA lesion initiating this response is the DSB (both accidental and programmed) (26), and as a result the number of g-H2AX foci scored approximates the number of DSBs induced (29).”
Pathways of DNA Double-Strand Break Repair during the Mammalian Cell Cycle (2003, methodology): “In the same study of Rothkamm and Lo ¨brich ( 49 ), the kinetics of the disappearance of foci closely resembled the kinetics of DSB repair, for both repair-proficient and DNA ligase IV-defective primary human fibroblasts.”
Persistent DNA damage signaling triggers senescence-associated inflammatory cytokine secretion (2009, background): “5 Gy of X-irradiation, which generates about 17 DSBs per nucleus”
γH2AX foci analysis for monitoring DNA double-strand break repair: Strengths, limitations and optimization (2010, background): “γH2AX foci analysis is one such assay that exploits an early step in the damage response, namely the phosphorylation of H2AX, a variant form of the histone, H2A.(2,3) Since phosphorylated H2AX, designated γH2AX, expands a large distance (Mbp) from the DSB site and since specific γH2AX antibodies are”