Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.

By J. Conde‐Vancells, E. Rodríguez‐Suárez, N. Embade, David Gil, R. Matthiesen, M. Valle, F. Elortza, Shelly C. Lu, J. Mato, Juan Manuel Falcón-Pérez. Journal of Proteome Research, 2008

605 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/0a3bcc26c70d1fc8c1a03035e0187860e1afc8d6

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. C. Théry
2. G. Raposo
3. Suresh Mathivanan
4. Hina Kalra
5. Richard J. Simpson
6. Marina Colombo
7. A. Llorente
8. H. Ji

In Molecular Biology

1. C. Théry
2. G. Raposo
3. Suresh Mathivanan
4. Richard J. Simpson
5. Marina Colombo
6. Hina Kalra
7. A. Llorente
8. H. Ji

Citations in the corpus

• Exosomes: extracellular organelles important in intercellular communication. (2010, background): “[34] Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM.”
• Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. (2014, methodology): “…of subcellular structures: Exosomes observed by cryo-EM (a technique in which samples are vitrified in liquid ethane to prevent the formation of ice crystals that can alter the ultrastructure of cells and membranes) have a round shape (Conde-Vancells et al. 2008, Raposo & Stoorvogel 2013).”
• As we wait: coping with an imperfect nomenclature for extracellular vesicles (2013, result): “However, more recent studies in other cell types, and with other techniques, have shown that the small vesicles can have a broader size range and density (13,14), and that the cup-shaped morphology was, in fact, an experimental artefact (15).”
• Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis (2019, background): “The presence of this protein has been established in hepatocyte derived exosomes [128], and therefore has the ability to be used as a marker to isolate liver derived exosomes.”
• Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. (2012, methodology): “These latter are thought to represent exosomes deformed during the preparative process, as they are not found when the more structurepreservative method of cryoelectron microscopy is used [14].”
• Exosomes: vehicles of intercellular signaling, biomarkers, and vectors of cell therapy. (2015, background): “Quickly frozen exosomes analyzed by cryo-electron microscopy have a perfectly round shape (34).”
• Extracellular vesicles: Exosomes, microvesicles, and friends (2013, neutral): “1 and not depicted; Conde-Vancells et al., 2008).”
• Introduction to Extracellular Vesicles: Biogenesis, RNA Cargo Selection, Content, Release, and Uptake (2016, neutral): “Comprehensive research has been done on the protein cargo of EVs, profiling the contents of different-sized vesicles produced by various cell types (Conde-Vancells et al. 2008; Demory Beckler et al. 2013; Gonzalez-Begne et al. 2009; Graner et al. 2009; Théry et al. 2001; Turiák et al. 2011).”
• Exosomes--vesicular carriers for intercellular communication. (2009, background): “Exosomes are between 40 and 100 nm in diameter, appear with a characteristic cup-shaped morphology (after negative staining) or as round well delimited vesicles as observed by transmission and cryo-electron microscopy, respectively [10].”
• Biological properties of extracellular vesicles and their physiological functions (2015, background): “For example, it has been shown that primary cultured hepatocytes are able to secrete EVs that, based on density, structure and composition, show many exosomal features (570).”
• Focus on Extracellular Vesicles: Introducing the Next Small Big Thing (2016, background): “, CD9, CD63, CD81, CD82), antigen presentation (MHC class molecules), heat shock proteins (HSP70, HSP90) and lipid-related proteins [124,131–133] are often identified in exosomes irrespective of the cell type of origin (Figure 3B).”
• Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A–C (2010, background): “The proteins found in our exosome preparation were compared with proteins identified previously in exosomes from other cell types (Barile et al., 2005; Segura et al., 2005; Aoki et al., 2007; Valadi et al., 2007; Conde-Vancells et al., 2008).”
• Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics (2020, background): “, N-linked glycans, alpha-2,6 sialic acid, mannose, and polylactosamine) overexpressed on exosomal membranes [99].”
• Exosome Theranostics: Biology and Translational Medicine (2018, neutral): “(Reproduced with permission from reference [36].”