Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis

By Laura M Doyle, Michael Zhuo Wang. Cells, 2019

2,722 citations recorded by the source corpus.

The use of extracellular vesicles, specifically exosomes, as carriers of biomarkers in extracellular spaces has been well demonstrated. Despite their promising potential, the use of exosomes in the clinical setting is restricted due to the lack of standardization in exosome isolation and analysis methods. The purpose of this review is to not only introduce the different types of extracellular vesicles but also to summarize their differences and similarities, and discuss different methods of exosome isolation and analysis currently used. A thorough understanding of the isolation and analysis methods currently being used could lead to some standardization in the field of exosomal research, allowing the use of exosomes in the clinical setting to become a reality.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ec3e8e822b12cec346d9035a9b37cb73b8ae4ed6

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Citations in the corpus

The exosome journey: from biogenesis to uptake and intracellular signalling (2021, background): “Background Extracellular vesicles (EVs) are released by all cells, prokaryotes and eukaryotes, and regulate intercellular communication in health and disease [1, 2].”
Clinical applications of stem cell-derived exosomes (2024, methodology): “19 In addition, cargo pro ﬁ ling is further subdivided into proteomic, lipidomic, and genomic analyses including western blotting, ELISA, ﬂ ow cytometry, mass spectroscopy, and PCR. 36 Since each of the above characterization methods has advantages and disadvantages, it is a universal practice to…”