Calpain-mediated proteolysis of talin regulates adhesion dynamics

By Santos J. Franco, M. Rodgers, B. Perrin, Jaewon Han, David A Bennin, D. Critchley, A. Huttenlocher. Nature Cell Biology, 2004

603 citations recorded by the source corpus.

Subfields: Biomaterials, Cell Biology, Immunology and Allergy

View Paper: https://www.semanticscholar.org/paper/f9964b285e9c4845a13baae7825bd8910fd534f7

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomaterials

1. J. Parsons

In Cell Biology

1. A. Horwitz
2. J. Parsons
3. M. Schwartz
4. D. Schlaepfer
5. D. Calderwood
6. S. Shattil
7. M. Frame
8. G. Gundersen

In Immunology and Allergy

1. J. Parsons
2. M. Schwartz
3. D. Calderwood
4. A. Horwitz
5. D. Schlaepfer
6. S. Shattil
7. Clare M. Waterman
8. E. Danen

Citations in the corpus

• ILK, PINCH and parvin: the tIPP of integrin signalling (2006, background): “Calpain-4 has been shown to cleave talin, and this cleavage is the rate-limiting step in the disassembly of focal adhesion”
• The role of focal-adhesion kinase in cancer — a new therapeutic opportunity (2005, background): “Indeed, recent work has shown that calpain-mediated cleavage of talin is a rate-limiting step in focal-adhesion turnover that is needed for cell migratio”
• Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase (2005, background): “, although recent studies implicate calpain in focal adhesion remodelling in migrating cell”
• Reconstructing and Deconstructing Agonist-Induced Activation of Integrin αIIbβ3 (2006, methodology): “PAC-1 binding to A5 cells transfected with either wildtype or calpain-resistant mutant GFP-talin (L432G) [36] in combination with Rap1A(G12V) and PAC-1 binding was assessed by flow cytometry, and protein expression was verified by western blotting. activation, and an ‘‘activated’’ fragment of talin ”
• Adhesion signaling – crosstalk between integrins, Src and Rho (2009, background): “The proteolytic cleavage of talin by calpain is important for cell-matrix adhesion turnover (Franco et al., 2004).”
• FAK promotes recruitment of talin to nascent adhesions to control cell motility (2012, background): “E1015A is that both FAK and talin are substrates of the calpain II protease involved in adhesion turnover (Franco et al., 2004; Chan et al., 2010).”
• Analysis of the myosinII-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation (2011, background): “Subunit of macromolecular complex (47) 1.”
• Cell adhesion: integrating cytoskeletal dynamics and cellular tension (2010, background): “Both talin 1 and the integrin β3 cytoplasmic domain have been identified as key calpain substrates in adhesion disassembly, although there are many others with a functional significance that is less well investigate”
• Activation, coactivation, and costimulation of resting human natural killer cells (2006, background): “Calpain-mediated cleavage of talin, a cytoskeletal component, produces a talin fragment that binds the cytoplasmic tail of integrin b chains, thereby inducing separation of the cytoplasmic tails and augmenting LFA-1 affinity (114, 117).”
• Integrin signalling at a glance (2009, background): “Proteolysis of talin by the intracellular protease calpain also enhances adhesion turnover (Franco et al., 2004) and calpain proteolysis of β-integrin tails modulates integrin signalling, favouring retraction rather than cell spreading (Flevaris et al., 2007).”
• Mechanical Integration of Actin and Adhesion Dynamics in Cell Migration (2010, background): “Alternative mechanisms for adhesion disassembly include calpainmediated proteolysis of talin (Franco et al. 2004) and microtubule-mediated adhesion relaxation (Kaverina et al. 1999, Krylyshkina et al. 2002).”