The nuclear RNase III Drosha initiates microRNA processing

By Yoontae Lee, Chiyoung Ahn, Jinju Han, Hyounjeong Choi, Jaekwang Kim, J. Yim, Junho Lee, P. Provost, O. Rådmark, Sunyoung Kim and 1 more. Nature, 2003

5,453 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f55b0476eae94742e77590fb2cb8bb54b661b95b

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Citations in the corpus

Processing of primary microRNAs by the Microprocessor complex (2004, background): “Initial cleavage is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleu”
A human snoRNA with microRNA-like functions. (2008, background): “…that are further processed to hairpin-structured miRNA precursors (pre-miRNAs) by the nuclear microprocessor complex containing the RNase III enzyme Drosha and its cofactor DGCR8 (Borchert et al., 2006; Denli et al., 2004; Gregory et al., 2004; Landthaler et al., 2004; Lee et al., 2003, 2004).”
TransmiR v2.0: an updated transcription factor-microRNA regulation database (2018, background): “On the one hand, there are difficulties to gain accurate locations of miRNA gene transcription start sites (TSSs) and therefore the locations of miRNA promoters, because the 5′ end of primary miRNA transcript is often rapidly sliced by Drosha in the nucleus (13).”
MicroRNA genes are transcribed by RNA polymerase II (2004, methodology): “Pre-miR-24-2 was prepared by in vitro processing as previously described (Lee et al, 2003).”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “Conversely, Drosha in the context of the microprocessor negatively regulates DGCR8 mRNA post-transcriptionally by cleaving an 88 nt hairpin located in the 5’ untranslated region (UTR) which destabilizes the transcript [89, 90].”
miR-499 regulates mitochondrial dynamics by targeting calcineurin and dynamin-related protein-1 (2011, background): “2282" language="eng" relation="no"> MicroRNAs are about 22 nucleotides long and act as negative regulators of gene expression by inhibiting mRNA translation or promoting mRNA degradatio”
Control of translation and mRNA degradation by miRNAs and siRNAs. (2006, background): “These are processed in the nucleus by a complex comprised of at least two components: the RNase III enzyme Drosha, and a protein called Pasha in Drosophila or DGCR8 in mammals (Lee et al. 2003; Denli et al. 2004; Gregory et al. 2004; Han et al. 2004; Landthaler et al. 2004).”
Development of microRNA therapeutics is coming of age (2014, neutral): “…70 nt pre-miRNA hairpin structures and then in the cytoplasm by the Dicer – TRBP complex to approximately 22 nt miRNA duplexes (Fig 1) (Bernstein et al , 2001; Grishok et al , 2001; Hutva´gner et al , 2001; Lee et al , 2003, 2004; Yi et al , 2003; Bohnsack et al , 2004; Denli et al , 2004).”
Human embryonic stem cells express a unique set of microRNAs. (2004, background): “These primary transcripts are first trimmed into approximately 70 nt stem-loop forms (called pre-miRNAs) by the RNase III type protein, Drosha, in the nucleus (Lee et al., 2003).”
The role of PACT in the RNA silencing pathway (2006, methodology): “In vitro processing of pre-miRNAs using FLAG-immunoprecipitates or recombinant human Dicer (Stratagene) was carried out as described previously (Lee et al, 2002, 2003).”
Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. (2006, background): “Once in the cytoplasm, miRNAs are cleaved further by the enzyme DICER (Lee et al., 2002, 2003), and this results in 17–24 nt miRNAs that are associated with a cellular complex that is similar to the RNA-induced silencing complex that participates in RNA interference (Hutvagner and Zamore, 2002).”