Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts

By M. Nakagawa, Michiyo Koyanagi, K. Tanabe, Kazutoshi Takahashi, T. Ichisaka, T. Aoi, K. Okita, Yuji Mochiduki, Nanako Takizawa, S. Yamanaka. Nature Biotechnology, 2008

2,906 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e11dc4c2ab3176e90cf19ae0fe426b93f4b0aca1

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Citations in the corpus

Satellite cells and the muscle stem cell niche. (2013, background): “Over the last five years, various types of differentiated somatic cells have been successfully induced into pluripotent stem cells (iPS cells) via a process involving transcription factor based reprogramming (371, 384, 515, 516, 582).”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “Consistent with c-Myc’s role as a facilitator of early stages of reprogramming, exogenous c-Myc can be omitted entirely from the reprogramming cocktail (Nakagawa et al. 2008; Wernig et al. 2008b), albeit at the cost of reduced reprogramming efficiency and speed.”
A fresh look at iPS cells. (2009, background): “In addition, Oct3/4 cannot be replaced by other Oct family members (Oct1 or Oct6) to generate iPS cells (Nakagawa et al., 2008).”
Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. (2010, background): “No increase was observed with SKO (not shown), possibly because of low efficacy of this combination in the human context ( Nakagawa et al., 2008; Wernig et al., 2008).”
Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 (2008, background): “Indeed, reactivation of the c-Myc retrovirus contributes to tumor formation in chimeric mice derived from iPS cell”
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. (2008, background): “Omission of c-Myc from the reprogramming cocktail further reduces the efficiency and delays the process (Nakagawa et al., 2008; Wernig et al., 2008).”
Non-coding RNAs as regulators of embryogenesis (2011, background): “The observation that MYC and KLF4 can be replaced by LIN28 and Nanog in iPS cell generatio”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “However, in the absence of c-Myc it takes longer to obtain reprogrammed colonies and the efficiency of the process is much lower (Nakagawa et al., 2008; Wernig et al., 2007).”
Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy (2011, background): “Most importantly, even with three-factor transduction, the first contracting patches emerge within 12 days, a much shorter period than the approximately three-week minimum required to generate iPSCs with three factor”
An extended transcriptional network for pluripotency of embryonic stem cells. (2008, background): “This unique target regulation by Myc may account for its capacity to enhance reprogramming, while also being dispensable as an exogenous factor (Nakagawa et al., 2007; Wernig et al., 2007a).”
The tumorigenicity of human embryonic and induced pluripotent stem cells (2011, methodology): “Recently, new methods have been developed to reprogramme human somatic cells without MYC”
Parkinson’s Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors (2009, background): “…we find that derivation of hiPSCs with three factors is more practical, since the infected fibroblast cultures are not overgrown by granulated, fast growing non-hiPSC colonies, as has been described previously for cultures infected with four factors (Nakagawa et al., 2008; Takahashi et al., 2007).”