Induced pluripotency: history, mechanisms, and applications.

By M. Stadtfeld, K. Hochedlinger. Genes & Development, 2010

831 citations recorded by the source corpus.

The generation of induced pluripotent stem cells (iPSCs) from somatic cells demonstrated that adult mammalian cells can be reprogrammed to a pluripotent state by the enforced expression of a few embryonic transcription factors. This discovery has raised fundamental questions about the mechanisms by which transcription factors influence the epigenetic conformation and differentiation potential of cells during reprogramming and normal development. In addition, iPSC technology has provided researchers with a unique tool to derive disease-specific stem cells for the study and possible treatment of degenerative disorders with autologous cells. In this review, we summarize the progress that has been made in the iPSC field over the last 4 years, with an emphasis on understanding the mechanisms of cellular reprogramming and its potential applications in cell therapy.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e62a4ed0205d1e6db14c89fbb03ba377bef05cea

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Citations in the corpus

Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines (2011, positive): “…is true for induced pluripotent stem (iPS) cell lines, which are obtained by reprogramming somatic cells using ectopic expression of the transcription factors OCT4, SOX2, KLF4, and C-MYC (Takahashi et al., 2007) or alternative reprogramming cocktails (reviewed in Stadtfeld and Hochedlinger, 2010).”
Control of Embryonic Stem Cell State (2011, background): “, 2008), the influence of extrinsic factors on pluripotency (Pera and Tam, 2010), and cellular reprogramming into ESC-like states (Hanna et al., 2010; Stadtfeld and Hochedlinger, 2010; Yamanaka and Blau, 2010).”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “…efficiency, expressed as number of AP-positive colonies per 100 cells transduced, was similar to the ones reported in the literature (0.01%-0.1% (Stadtfeld and Hochedlinger, 2010)(Figure 4.6C), although this depends on several factors such as transduction efficiency and type and passage number…”
Understanding multicellular function and disease with human tissue-specific networks (2015, positive): “For example, developmental programs are known to be controlled by broadly-expressed transcription factors that, in specific combinations, regulate cell-type-specific gene expression 5,22,23 and have the potential to force cell-lineage conversions 24,25 .”
Emerging trends and new developments in regenerative medicine: a scientometric update (2000 – 2014) (2014, methodology): “(2014) 14(9) 1309 Takahashi2007 [35] and Stadtfeld2008 [9].”
A double take on bivalent promoters. (2013, background): “Somatic cells such as fibroblasts can be reprogrammed to so-called induced pluripotent stem cells (iPSCs) by expression of select pluripotency factors (Takahashi and Yamanaka 2006; Stadtfeld and Hochedlinger 2010).”