Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis

By Zhengyan Feng, Yanfei Mao, N. Xu, Botao Zhang, Peng-lian Wei, Dong-Lei Yang, Zhen Wang, Zhengjing Zhang, Rui Zheng, Lan Yang and 3 more. Proceedings of the National Academy of Sciences of the United States of America, 2014

671 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/d1ed08d9e35fd8eafcae91173290075140ddd30f

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Citations in the corpus

• Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922 (2016, background): “This phenomenon has been reported in rice [29, 34, 35, 38], Arabidopsis [74], wheat [47], tomato [49] and maize [42].”
• Target specificity of the CRISPR-Cas9 system (2014, unknown): “So far relatively high coverage (30-60X) of WGS has been performed in single clones of Cas9 treated cells in a variety of species, including worm [48], Arabidopsis [49], rice [50], and human pluripotent stem cells [51,52].”
• A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. (2015, background): “This study and other reports (Feng et al., 2014; Zhang et al., 2014) have shown that theediting efficiency andmutation types induced by CRISPR/Cas9 systems are very different in the first transgenic generation of rice andArabidopsis.”
• CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. (2019, background): “An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing.”
• A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “Agrobacterium-mediated transformation is a routine method used to generate transgenic plants, and a few binary vectors have been developed to deliver the CRISPR/Cas9 system into plant genomes via this method [15,20,23,24,33-40].”
• Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA[OPEN] (2015, methodology): “Proc Natl Acad Sci USA 99: 11975–11980 Halfter U, Morris PC, Willmitzer L (1992) Gene targeting in Arabidopsis thaliana.”
• The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “benthamiana, wheat and sweet orange, or in an independent study involving rice, when potential off-target sites with at least a conserved 12-nt seed sequence were investigated (Feng et al., 2014; Jia and Wang, 2014; Li et al., 2013; Nekrasov et al., 2013; Shan et al., 2013; Upadhyay et al., 2013; Zh”
• A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, methodology): “After initial reports, many studies quickly announced successful and effective CRISPR/Cas9-mediated genome editing in plants (Feng et al., 2013, 2014; Jiang et al., 2013; Li et al., 2013; Mao et al., 2013; Miao et al., 2013; Nekrasov et al., 2013; Shan et al., 2013b; Xie and Yang, 2013; Fauser et…”
• Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System1 (2014, background): “In contrast, several recent studies have shown high specificity of CRISPR/Cas9 in plants (Feng et al., 2014; Gao and Zhao, 2014; Zhang et al., 2014).”
• Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, background): “However, transgenic lines expressing CRISPR/Cas9 have mainly been mosaic in the first generation (T1), indicating that CRISPR/Cas9-induced mutations in Arabidopsis occurred after the first embryonic cell division [20, 22, 25, 26, 29, 35].”
• Interspecific Hybridization for Brassica Crop Improvement (2019, background): “Although this technology is still in its early stages, its application has been demonstrated not only in model species such as Arabidopsis thaliana [169], but also in crops such as tomato (Solanum lycopersicum)[170] and wheat (Triticum aestivum)[171].”
• The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation. (2014, methodology): “This is in contrast to the lack of any homozygotes or bi-alleles in the T1 generation of CRISPR/Cas9 transgenic Arabidopsis plants (Feng et al., 2014).”
• Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, unknown): “…watermelon and mushroom (Jiang et al., 2013; Li et al., 2013; Mao et al., 2013; Nekrasov et al., 2013; Shan et al., 2013a; Brooks et al., 2014; Feng et al., 2014; Jia and Wang, 2014; Sugano et al., 2014; Fan et al., 2015; Lawrenson et al., 2015; Li et al., 2015; Svitashev et al., 2015; Wang…”