The CRISPR/Cas9 system for plant genome editing and beyond.

By L. Bortesi, R. Fischer. Biotechnology Advances, 2015

1,061 citations recorded by the source corpus.

Targeted genome editing using artificial nucleases has the potential to accelerate basic research as well as plant breeding by providing the means to modify genomes rapidly in a precise and predictable manner. Here we describe the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a recently developed tool for the introduction of site-specific double-stranded DNA breaks. We highlight the strengths and weaknesses of this technology compared with two well-established genome editing platforms: zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). We summarize recent results obtained in plants using CRISPR/Cas9 technology, discuss possible applications in plant breeding and consider potential future developments.

Subfields: Insect Science, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/3fa806418924e796feb2be1025d3eb4f018953af

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Insect Science

1. Yong‐Bi Fu

In Molecular Biology

1. Heribert Hirt
2. Yong‐Bi Fu
3. S. Bhattacharjee
4. E. A. Ibrahim

In Plant Science

1. Heribert Hirt
2. L. Guarino
3. Yong‐Bi Fu
4. H. Dempewolf
5. S. Bhattacharjee
6. E. A. Ibrahim

Citations in the corpus

• The heat‐shock protein/chaperone network and multiple stress resistance (2017, background): “In this regard, genome editing by CRISPR-CAS9 would be cleaner and faster (Bortesi and Fischer, 2015).”
• Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology. (2016, methodology): “Cas9/ sgRNA editing has been demonstrated in various plant families, e.g. Cruciferae, Solanaceae, Poaceae and Fabaceae (Belhaj et al., 2013; Bortesi and Fischer, 2015; Jacobs et al., 2015; Xie et al., 2014), since it was first reported in 2013 (Li et al., 2013; Nekrasov et al., 2013).”
• Past and Future Use of Wild Relatives in Crop Breeding (2017, background): “The advent of genome editing is predicted to revolutionize plant breeding (Voytas 2013; Bortesi and Fischer, 2015; Khatodia et al., 2016).”
• The Vulnerability of Plant Genetic Resources Conserved Ex Situ (2017, positive): “, 2011) thanks to advances in biotechnology such as genome editing (Bortesi and Fischer, 2015).”