Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement

By Hui Zhang, Jinshan Zhang, Zhaobo Lang, J. Botella, Jian‐Kang Zhu. 2017

116 citations recorded by the source corpus.

ABSTRACT Genome editing technologies are powerful tools for studying gene function and for crop improvement. The technologies rely on engineered endonucleases to generate double stranded breaks (DSBs) at target loci. The DSBs are repaired through the error-prone non-homologous end joining (NHEJ) and homology-directed repair (HDR) pathways in cells, resulting in mutations and sequence replacement, respectively. In the widely used CRISPR/Cas9 system, the endonuclease Cas9 is targeted by a CRISPR small RNA to DNA sequence of interest. In this review, we describe the four available types of genome editing tools, ZFN, TALEN, CRISPR/Cas9 and CRISPR/Cpf1, and show their applications in functional genomics research and precision molecular breeding of crops.

Subfields: Aging, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/3757ca5941f0b7b9e83c2a1e913360d06595e4f0

Citations in the corpus

• The WRKY Transcription Factor Family in Model Plants and Crops (2017, unknown): “Gene editing tools such as CRISPR (Clustered Regularly Interspaced Palindromic Repeats)-Cas9 (Songstad et al., 2017; Zhang et al., 2017) have been rapidly developed in the past years.”