A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.

By Xingliang Ma, Qunyu Zhang, Qinlong Zhu, Wei Liu, Yan Chen, Rong Qiu, B. Wang, Zhongfang Yang, Heying Li, Yuru Lin and 10 more. Molecular Plant, 2015

1,963 citations recorded by the source corpus.

Subfields: Insect Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5e36c6da24a6c2a98e1a6e78622a501d69aa1175

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In Insect Science

1. Caixia Gao
2. Jian‐Kang Zhu

In Molecular Biology

1. Caixia Gao
2. Kunling Chen
3. Yanpeng Wang
4. Jian‐Kang Zhu
5. Daniel F. Voytas

Citations in the corpus

• Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922 (2016, methodology): “The integrated sgRNA expression cassettes were then amplified by nested PCR [38] using U-F/Reverse adapter primers (E922-RS1, E922-RS2 and E922-RS3) and Forward adapter primers (E922-FS1, E922-FS2 and E922-FS3)/gR-R for the first round, and the corresponding site-specific primers Pps-GGL/Pgs-GG2, Pp”
• Genome Engineering Using the CRISPR Cas9 System (2019, methodology): “TALENS was used in 2010 to edit genome of yeast and later on human cells [1,3,4,8].”
• Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, methodology): “Several approaches have been developed to express multiple sgRNAs, such as Golden Gate assembly, polycistronic tRNA–gRNA system, selfcleaving ribozyme flanked gRNAs and target-adaptor ligation (Gao and Zhao, 2014; Lowder et al., 2015; Ma et al., 2015; Xie et al., 2015).”
• CRISPR/Cas9-Induced Mutagenesis of Semi-Rolled Leaf1,2 Confers Curled Leaf Phenotype and Drought Tolerance by Influencing Protein Expression Patterns and ROS Scavenging in Rice (Oryza sativa L.) (2019, methodology): “The CRISPR/Cas9 system has also been employed for the simultaneous editing of multiple genes [22], as well as target gene repression and activation [23].”
• Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, methodology): “Since methods have been developed to assemble multiple sgRNAs [26, 37, 54, 56, 57], highly efficient expression of multiple sgRNAs in a cell has not been a problem.”
• A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, methodology): “While we were preparing our article, Xing et al. (2014) and Ma et al. (2015) reported a CRISPR/Cas9 toolkit for targetedmutagenesis in plants.”
• CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. (2019, methodology): “Rice with low amylose content, and thus improved rice eating and cooking quality, was generated by knockout ofWaxy via CRISPR/Cas9 (100, 185).”
• Past and Future Use of Wild Relatives in Crop Breeding (2017, background): “, chromosomal fragments), are becoming increasingly feasible (Lowder et al., 2015; Ma et al., 2015).”