Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation
By Zhiping Wang, Hui-Li Xing, Li Dong, Hai-Yan Zhang, Chun-Yan Han, Xue-Chen Wang, Qi-jun Chen. Genome Biology, 2015
882 citations recorded by the source corpus.
Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of Cas9 and obtained non-mosaic T1 mutants for multiple target genes with high efficiency. Comparisons of 12 combinations of eight promoters and two terminators found that the efficiency of the egg cell-specific promoter-controlled CRISPR/Cas9 system depended on the presence of a suitable terminator, and the composite promoter generated by fusing two egg cell-specific promoters resulted in much higher efficiency of mutation in the T1 generation compared with the single promoters.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/1f4b732732e9ca12858bea4ebe35dcf6cb77a604
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Citations in the corpus
- Genome Editing of Plants (2017, background): “express the nuclease preferentially in the germline (Wang et al., 2015; Mao et al., 2016).”