Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System1

By Christopher Brooks, V. Nekrasov, Z. Lippman, J. Van Eck. Plant Physiology, 2014

683 citations recorded by the source corpus.

Subfields: Business and International Management, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/3324e7de1b38bda020ab910d91d17fea41d78b73

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In Business and International Management

Z. Lippman

In Molecular Biology

In Plant Science

Citations in the corpus

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA[OPEN] (2015, background): “Testing of S. pyogenes Cas9-gRNA in a variety of plant species has resulted in extremely low frequency of nonspecific DNA cleavage and therefore, high specificity of the system (Feng et al., 2013; Shan et al., 2013; Brooks et al., 2014; Zhang et al., 2014).”
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, result): “Similar results were reported in tomato [33] and”
Interspecific Hybridization for Brassica Crop Improvement (2019, background): “Although this technology is still in its early stages, its application has been demonstrated not only in model species such as Arabidopsis thaliana [169], but also in crops such as tomato (Solanum lycopersicum)[170] and wheat (Triticum aestivum)[171].”
Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing (2022, positive): “CRISPR-Cas9 mutagenesis was performed as described previously [56].”
Automated assembly scaffolding elevates a new tomato system for high-throughput genome editing (2021, positive): “CRISPR-Cas9 mutagenesis, plant transformation, and identification of mutant alleles CRISPR-Cas9 mutagenesis was performed as described previously [45].”
Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922 (2016, background): “In particular, CRISPR/Cas9 has been demonstrated to be the most effective SSN thus far and has been used for genome editing in major crops such as rice [29–41], maize [42–45], wheat [39, 46, 47], sorghum [30], tomato [48, 49], soybean [50–52] and potato [53].”
Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, background): “…flax, petunia, citrus, watermelon and mushroom (Jiang et al., 2013; Li et al., 2013; Mao et al., 2013; Nekrasov et al., 2013; Shan et al., 2013a; Brooks et al., 2014; Feng et al., 2014; Jia and Wang, 2014; Sugano et al., 2014; Fan et al., 2015; Lawrenson et al., 2015; Li et al., 2015; Svitashev…”
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, background): “In fact, simultaneous targeting of genes using multiple gRNAs has been demonstrated repeatedly in plants (Li et al., 2013; Mao et al., 2013; Upadhyay et al., 2013; Brooks et al., 2014; Gao et al., 2015).”
Major impacts of widespread structural variation on gene expression and crop improvement in tomato (2020, neutral): “CRISPR-Cas9 mutagenesis, plant transformation, and selection of mutant alleles CRISPR-Cas9 mutagenesis and generation of transgenic tomato was performed following our standard protocol (Brooks et al., 2014).”
Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology. (2016, background): “…the CEC1-1 line, the same mutations were observed in the T1 generation, which implies a heterozygous VC 2016 BSPP AND JOHN WILEY & SONS LTD MOLECULAR PLANT PATHOLOGY (2016) 17 (7 ) , 1140–1153 mono-allellic CEC1-1 T0 plant, as observed in tomato (Brooks et al., 2014) and rice (Zhang et al., 2014).”
Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing. (2017, methodology): “Obtaining homozygous mutants from CRISPR/Cas9 in firstgeneration transgenics is rare (Brooks et al., 2014; Svitashev et al., 2015; Zhang et al., 2014), and the weak T0-1 phenotype was surprising considering the 1.”
The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “It was also shown in Arabidopsis, rice and tomato that the genetic changes induced by Cas9/gRNA were present in the germ line and segregated normally in subsequent generations without further modifications (Brooks et al., 2014; Fauser et al., 2014; Feng et al., 2014; Jia et al., 2014; Schiml et al.,”