The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation.

By Hui Zhang, Jinshan Zhang, Peng-lian Wei, Botao Zhang, Feng Gou, Zhengyan Feng, Yanfei Mao, Lan Yang, Heng Zhang, N. Xu and 1 more. Plant Biotechnology Journal, 2014

763 citations recorded by the source corpus.

Subfields: Business and International Management, Insect Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b6d1230bb36abccd96b410a5f776f650d9362f77

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Citations in the corpus

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology. (2016, background): “Alternatively, the T0 plant was chimeric, in which expression of Cas9/sgRNA2 occurred in the germ cell line, as observed in rice (Zhang et al., 2014).”
A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “Agrobacterium-mediated transformation is a routine method used to generate transgenic plants, and a few binary vectors have been developed to deliver the CRISPR/Cas9 system into plant genomes via this method [15,20,23,24,33-40].”
The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “These studies provided the first comparative data concerning aspects such as mutation efficiency, cleavage specificity, the resolution of locus structure and the potential to create large chromosomal deletions and also demonstrated that gRNAs can be expressed under the control of diverse promoters, ”
Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing. (2017, background): “Obtaining homozygous mutants from CRISPR/Cas9 in firstgeneration transgenics is rare (Brooks et al., 2014; Svitashev et al., 2015; Zhang et al., 2014), and the weak T0-1 phenotype was surprising considering the 1.”
Gene Pyramiding for Sustainable Crop Improvement against Biotic and Abiotic Stresses (2020, background): “Low temperature tolerant genes (OsRAN1) and QTLs have also been identified in many plants for further use in cold tolerance development in crop varieties through molecular marker breeding tools [46-48].”
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. (2015, background): “…current plant CRISPR/Cas9 vector systems can only target one or few genomic sites (Jiang et al., 2013a, 2013b; Li et al., 2013; Mao et al., 2013; Shan et al., 2013a, 2013b; Xie and Yang, 2013; M Fauser et al., 2014; Feng et al., 2014; Xing et al., 2014; Zhang et al., 2014; Zhou et al., 2014).”
Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922 (2016, background): “This phenomenon has been reported in rice [29, 34, 35, 38], Arabidopsis [74], wheat [47], tomato [49] and maize [42].”
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, neutral): “…pYPQ154; Fauser et al., 2014; Schiml et al., 2014), human codon-optimized Cas9 (hSpCas9, pYPQ158; Feng et al., 2013, 2014; Mao et al., 2013; Zhang et al., 2014), and a plant codonoptimized Cas9 harboring enriched guanine-cytosine content within key 59 coding regions (Cas9p, pYPQ167; Ma et…”
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, background): “ation, indicating that the target genes were edited in the transformed embryogenic cells before the first cell division [28].”
Target specificity of the CRISPR-Cas9 system (2014, neutral): “[50], and human pluripotent stem cells [51,52].”
Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System1 (2014, background): “…Arabidopsis (Arabidopsis thaliana; Li et al., 2013) and Nicotiana benthamiana (Nekrasov et al., 2013), there have also been reports for three crop species, rice (Oryza sativa; Zhang et al., 2014), sorghum (Sorghum bicolor; Jiang et al., 2013), and wheat (Triticum aestivum; Wang et al., 2014).”
Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, methodology): “off-target effects are limited in CRISPR–Cas9 edited plants (Zhang et al., 2014; Zhang et al., 2016b).”
CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool (2015, background): “…literally all (model) organisms with the emergence of engineered nucleases like transcription activator-like effector nucleases (TALEN) [1], Zinc-finger nucleases (ZFN) [2] or the RNA guided nucleases [3] facilitating the introduction of a double strand break (DSB) at any locus of choice [4 – 9].”