Target specificity of the CRISPR-Cas9 system

By Xuebing Wu, Andrea J. Kriz, Phillip A. Sharp. Quantitative Biology, 2014

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The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

Subfields: Aging, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/01e95b0e34b20edd432f6f6d0395aa3b4b29a509