MicroRNAs in plants.

By B. Reinhart, E. Weinstein, M. W. Rhoades, B. Bartel, David P. Bartel. Genes & Development, 2002

1,882 citations recorded by the source corpus.

MicroRNAs (miRNAs) are an extensive class of ~22-nucleotide noncoding RNAs thought to regulate gene expression in metazoans. We find that miRNAs are also present in plants, indicating that this class of noncoding RNA arose early in eukaryotic evolution. In this paper 16 Arabidopsis miRNAs are described, many of which have differential expression patterns in development. Eight are absolutely conserved in the rice genome. The plant miRNA loci potentially encode stem-loop precursors similar to those processed by Dicer (a ribonuclease III) in animals. Mutation of an Arabidopsis Dicer homolog, CARPEL FACTORY, prevents the accumulation of miRNAs, showing that similar mechanisms direct miRNA processing in plants and animals. The previously described roles of CARPEL FACTORY in the development of Arabidopsis embryos, leaves, and floral meristems suggest that the miRNAs could play regulatory roles in the development of plants as well as animals.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/b7f38626c1ffbc8acfcc6a4d4655c796debe6ea4

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Citations in the corpus

Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs[OPEN] (2013, background): “DCL1 is responsible for miRNA biogenesis from most MIR genes (Park et al., 2002; Reinhart et al., 2002).”
MicroRNA functions in animal development and human disease (2005, methodology): “…have been identified in worms, flies, fish, frogs, mammals and flowering plants using molecular cloning and bioinformatics prediction strategies (LagosQuintana et al., 2001; Lau et al., 2001; Lee and Ambros, 2001; Lim et al., 2003a; Llave et al., 2002; Reinhart et al., 2002; Watanabe et al., 2005).”
A biochemical framework for RNA silencing in plants. (2003, background): “In addition to siRNAs, another class of small RNAs, microRNAs (miRNAs), has been detected in plants (Llave et al. 2002a; Park et al. 2002; Reinhart et al. 2002).”
Computational and experimental identification of C. elegans microRNAs. (2003, background): “Recent evidence from Arabidopsis thaliana indicates that miRNAs are also expressed in plants (Reinhart et al., 2002).”
Post-transcriptional small RNA pathways in plants: mechanisms and regulations. (2006, background): “DCL1 has both Drosha and Dicer functions and was found in the nucleus (Park et al. 2002; Reinhart et al. 2002; Papp et al. 2003; Kurihara and Watanabe 2004).”
MicroRNA-Directed Regulation of Arabidopsis AUXIN RESPONSE FACTOR17 Is Essential for Proper Development and Modulates Expression of Early Auxin Response Genesw⃞ (2005, methodology): “Many miRNAs isolated from the dicot Arabidopsis thaliana are conserved in the monocot rice (Oryza sativa) and in other plants, implying conserved evolutionary roles for plant miRNAs (Reinhart et al., 2002; Floyd and Bowman, 2004; Jones-Rhoades and Bartel, 2004; Sunkar and Zhu, 2004; Wang et al., 200”
Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step. (2004, background): “Sequences for miRNA* probes were predicted from miRNA precursor structures (Llave et al. 2002a; Reinhart et al. 2002).”
Human MicroRNA Targets (2004, background): “…transcripts containing short double-stranded RNA hairpins, named microRNAs (miRNAs), were identified in plants and animals (Lee et al. 1993; Reinhart et al. 2000, 2002; Lagos-Quintana et al. 2001, 2002, 2003; Lau et al. 2001; Lee and Ambros 2001; Llave et al. 2002a; Mette et al. 2002;…”
Microarray analysis of microRNA expression in the developing mammalian brain (2004, neutral): “elegans, plants, Drosophila melanogaster and mammals [6-18].”
RNA Interference: Biology, Mechanism, and Applications (2003, background): “Bartel’s group (181) has also shown that caf1 (dicer homologue) mutants of A.”
DNA methylation mediated by a microRNA pathway. (2010, background): “Plants lack Drosha homologs, and both processing steps are catalyzed by a Dicer-like enzyme (DCL1) in the nucleus (Kurihara et al., 2006; Kurihara and Watanabe, 2004; Park et al., 2002; Reinhart et al., 2002).”
Small RNAs and Their Roles in Plant Development (2009, background): “A MIR gene is transcribed by Pol II into a capped and polyadenylated pri-miRNA, which is processed into the stem-loop precursor, pre-miRNA, by a DCL protein, usually DCL1 in Arabidopsis (Park et al. 2002, Reinhart et al. 2002, Xie et al. 2005a).”
Endogenous siRNAs Derived from a Pair of Natural cis-Antisense Transcripts Regulate Salt Tolerance in Arabidopsis (2005, positive): “siRNAs were first identified in plants undergoing posttranscriptional gene silencing (PTGS; Hamilton and Baulcombe, 1999), and subsequently diverse sets of endogenous siRNAs have been found in plants and animals (Llave et al., 2002; Sunkar and Zhu, 2004; Ambros, 2003; Aravin et al., 2003; Tang et al”
Evidence that microRNA precursors, unlike other non-coding RNAs, have lower folding free energies than random sequences (2004, methodology): “However, in plant genomes the miRNA precursor sequences are often much longer (Llave et al., 2002; Reinhart et al., 2002).”
P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction. (2003, background): “In contrast, miR171 in dcl1-7 and dcl1-9 plants accumulated to less than 10% of normal levels (Figure 3D, lanesinflorescence tissue from TuMV-infected plants, accu- mulation of the cleavage product from both SCL6-III 9–11), as expected from previous studies (Reinhart et al., 2002).”