Characterization of Dicer-deficient murine embryonic stem cells.

By E. Murchison, J. Partridge, O. Tam, Sihem Cheloufi, Gregory J. Hannon. Proceedings of the National Academy of Sciences of the United States of America, 2005

818 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/98907ebedc1dd3849be620f0b43606e1e823e5f4

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. David P. Bartel
2. Phillip A. Sharp
3. Scott M. Hammond
4. Margaret S. Ebert
5. Nikolaus Rajewsky
6. Richard A. Young
7. Gregory J. Hannon
8. S. Baskerville

In Molecular Biology

1. David P. Bartel
2. Richard A. Young
3. Phillip A. Sharp
4. Scott M. Hammond
5. Nikolaus Rajewsky
6. Margaret S. Ebert
7. Gregory J. Hannon
8. D. Reinberg

Citations in the corpus

• MicroRNA sponges: progress and possibilities. (2010, background): “A sensitized background of DGCR8 and/or Dicer heterozygosity, which 2048 RNA, Vol. 16, No. 11 shows partially reduced levels for some miRNAs (Murchison et al. 2005; Wang et al. 2007), might enhance the loss of function.”
• Impaired microRNA processing enhances cellular transformation and tumorigenesis (2007, background): “Recent work with loss-of-function alleles of Dicer1 in mammals has described defects in processes potentially unrelated to miRNAs, such as silencing of heterochromatic regions and chromosome segregatio”
• A MicroRNA Feedback Circuit in Midbrain Dopamine Neurons (2007, methodology): “An ES cell line was obtained that conditionally expresses Dicer enzyme by harboring LoxP recombinase sites that flank both chromosomal copies of the Dicer gene (floxed Dicer alleles; Murchison et al. 2005 ).”
• Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure (2008, background): “Generation and genotyping of Dicer floxed (Dicerflox/flox) mice (31) and -MHC-Cre (MHCCre/ ) mice (13) have been described.”
• Epigenetic inheritance during the cell cycle (2009, background): “Whereas experimental evidence substantiates this model in yeast, whether RNAi is involved in heterochromatin maintenance in mammals is unclea”
• MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. (2009, neutral): “Murine ESCs with genetic deletion of key miRNA processing enzymes Dicer (Kanellopoulou et al., 2005; Murchison et al., 2005) or DGCR8 (Wang et al.”
• DNA methylation: The nuts and bolts of repression (2007, result): “There are contradicting results, however, Murchison et al. (2005) and further research need to be done to show that this mechanism occurs in mammals.”
• DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal (2007, background): “In addition, Dicer1 knockout ES cells seem to have a more profound initial proliferation defect that is overcome over time, presumably due to additional genetic event”
• Non-coding RNAs as regulators of embryogenesis (2011, background): “, and Dicer1 as well as Dgcr8 mutant mouse ES cells show severe growth and differentiation defect”
• Cell type–specific delivery of siRNAs with aptamer-siRNA chimeras (2006, background): “5a , bottom panels), as 21- to 23-nt long siRNAs have been shown to bypass the Dicer ste”
• Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes. (2006, background): “Although siRNA-mediated DNA modification and chromatin remodeling are controversial in mammals (Morris et al. 2004; Park et al. 2004; Svoboda et al. 2004b; Kanellopoulou et al. 2005; Murchison et al. 2005), it is tempting to speculate that differential expression of retrotransposons and subsequent p”
• MicroRNA control of signal transduction (2010, background): “Remarkably, however, inhibition of miRNA biogenesis (for example, through the ablation of Dicer) clearly reveals that miRNAs are essential for a wide array of biological processes, including control of proliferative homeostasis, differentiation or embryonic stemnes”
• MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. (2008, neutral): “Indeed, ES cells lacking Dicer or Drosha, and therefore most mature miRNAs, cannot differentiate into most lineages (Kanellopoulou et al., 2005; Murchison et al., 2005; Wang et al., 2007).”
• A human snoRNA with microRNA-like functions. (2008, methodology): “Total RNA from mouse embryonic stem (ES) cells carrying homozygous or heterozygous Dicer deletions (Murchison et al., 2005) was analyzed for the presence of ACA45 small RNAs by semiquantitative real-time PCR (qRT-PCR) (Figure 3F).”