Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells

By Chad A. Cowan, Jocelyn Atienza, D. Melton, K. Eggan. Science, 2005

968 citations recorded by the source corpus.

Subfields: Molecular Biology, Public Health, Environmental and Occupational Health

View Paper: https://www.semanticscholar.org/paper/977d3b54f4723f4096731ff7d3639fc2d4699e5d

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Citations in the corpus

Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. (2007, background): “Reprogramming of cells by nuclear transfer (Wakayama et al., 1998; Wilmut et al., 1997) and cell fusion ( Cowan etal.,2005; Tadaetal.,2001) allowsforthere-establishment of a pluripotent state in a somatic nucleus (Hochedlinger and Jaenisch, 2006).”
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (2006, background): “Somatic cells can be reprogrammed by transferring their nuclear contents into oocytes (Wilmut et al., 1997) or by fusion with ES cells (Cowan et al., 2005; Tada et al., 2001), indicating that unfertilized eggs and ES cells contain factors that can confer totipotency or pluripotency to somatic cells.”
Epigenetic reprogramming and induced pluripotency (2009, background): “…and induced pluripotency Konrad Hochedlinger1 and Kathrin Plath2 1Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, 185 Cambridge Street, Boston, MA 02114, USA.”
Strategies and new developments in the generation of patient-specific pluripotent stem cells. (2007, background): “More recently, reprogramming by fusion with human ES cells was reported (Cowan et al., 2005; Yu et al., 2006).”
Proliferation, survival and metabolism: the role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination (2016, background): “Differentiated somatic cells can be reprogrammed back to an embryonic-like pluripotent state, either by nuclear transfer, cell fusion or the delivery of certain factors, including transcription factors and small molecule inhibitors (Wilmut et al., 1997; Cowan et al., 2005; Takahashi and Yamanaka, 20”
Nuclear reprogramming to a pluripotent state by three approaches (2010, background): “Cowan and colleagues extended this work by showing that nuclear reprogramming of human somatic cells can be achieved by fusing them with human ES cells in tetraploid hybrids in a 1:1 rati”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “However, all of the pluripotent cell lines tested so far (ESCs, ECCs, and EGCs) have been shown to induce pluripotency in somatic cells after cellular fusion, demonstrating that they harbor dominant reprogramming activities (Tada et al. 1997, 2001; Cowan et al. 2005).”
Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. (2008, background): “Even though epigenetic reprogramming of a somatic genome to an embryonic state by nuclear transfer or fusion of ES cells with somatic cells has become a widely used procedure in various mammalian species over the last decade (Cowan et al., 2005; Kato et al., 1998; Polejaeva et al., 2000; Tada et al.”
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. (2008, background): “…can be achieved by nuclear transfer into oocytes (Wakayama et al., 1998; Wilmut et al., 1997), by cell fusion between ES cells and somatic cells (Cowan et al., 2005; Tada et al., 2003), and by the ectopic expression of transcription factors in somatic cells (Takahashi et al., 2007; Takahashi…”
Reprogramming of human somatic cells to pluripotency with defined factors (2008, background): “Of note, panel c contains all three germ layers: (1) glycogenated squamous epithelium, (2) immature cartilage, (3a) glandular tissue with surrounding stromal elements, and (3b) another small gland.”
An extended transcriptional network for pluripotency of embryonic stem cells. (2008, background): “Pluripotency may be imposed on somatic cells following their fusion with ES cells (Cowan et al., 2005), and one transcription factor specifically expressed in ES cells, Nanog, facilitates fusion-induced pluripotency (Silva et al.”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, result): “Similar results can be obtained by fusion with mES cells (Tada et al., 2001) and hES cells (Cowan et al., 2005; Yu et al., 2006).”