Nuclear reprogramming to a pluripotent state by three approaches

By S. Yamanaka, Helen M. Blau. Nature, 2010

774 citations recorded by the source corpus.

The stable states of differentiated cells are now known to be controlled by dynamic mechanisms that can easily be perturbed. An adult cell can therefore be reprogrammed, altering its pattern of gene expression, and hence its fate, to that typical of another cell type. This has been shown by three distinct experimental approaches to nuclear reprogramming: nuclear transfer, cell fusion and transcription-factor transduction. Using these approaches, nuclei from 'terminally differentiated' somatic cells can be induced to express genes that are typical of embryonic stem cells, which can differentiate to form all of the cell types in the body. This remarkable discovery of cellular plasticity has important medical applications.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c9fb30cd9f322147ed3e72ea763bf1b1d02de124

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Citations in the corpus

Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, neutral): “We focus here on unresolved or controversial issues of direct reprogramming because nuclear transfer and cell fusion approaches have been extensively reviewed elsewhere (Jaenisch and Young, 2008; Yamanaka and Blau, 2010).”
The repair Schwann cell and its function in regenerating nerves (2016, background): “The other main MAPK signalling pathways, p38 and JNK, are also activated by nerve injury, and all three MAPK pathways have been implicated in promoting de-differentiation of myelin cells (Myers et al. 2003; Parkinson et al. 2004; Yang et al. 2012).”
Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration (2011, methodology): “How this has been achieved by a range of different approaches was recently reviewed in Ref”
DNA Demethylation Dynamics (2011, background): “Nonetheless, nuclear reprogramming shows that perturbations are possible (Jullien et al., 2011; Yamanaka and Blau, 2010).”
Control of Embryonic Stem Cell State (2011, background): “, 2008), the influence of extrinsic factors on pluripotency (Pera and Tam, 2010), and cellular reprogramming into ESC-like states (Hanna et al., 2010; Stadtfeld and Hochedlinger, 2010; Yamanaka and Blau, 2010).”
Normal and neoplastic nonstem cells can spontaneously convert to a stem-like state (2011, background): “Spontaneous dedifferentiation in vivo may involve the reactivation of one or more of the described pluripotency factors (Oct4, Klf4, c-myc, and Sox-2) (32).”