Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells

By J. Draper, Kath Smith, P. Gokhale, H. Moore, E. Maltby, Julie A. Johnson, L. Meisner, T. Zwaka, James A. Thomson, P. Andrews. Nature Biotechnology, 2004

1,058 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/86384ef45498e55dbfcd7f2097313a9e334ac4e8

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

Feeder-independent culture of human embryonic stem cells (2006, result): “Taken together, these karyotype results are comparable to what we have previously observed with fibroblast coculture and enzymatic dissociatio”
Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells (2005, positive): “Abnormalities involving chromosomes 9, 5, and 19 were rarely observed at late passages (p80-p115) confirming recent reports suggesting that hESCs are susceptible to genetic anomalies (Draper et al., 2004).”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “Regular targeting approaches in human ESCs are quite inefficient, and may result in karyotypic abnormalities due to extensive culturing (Draper et al. 2004).”
Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells (2005, methodology): “ES cells in UM + bFGF + noggin have normal karyotype H1 cells cultured in UM plus bFGF and noggin for 5 passages, H9 for 33 passages and H14 for 19 passages were karyotyped by standard G-banding, and chromosomes 12 and 17 (ref”
Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes (2008, background): “Notably, karyotypic abnormalities involving chromosomes 17 and/or 20 are frequently detected in hES cells passaged enzymatically as single cell”
Testicular germ-cell tumours in a broader perspective (2005, background): “show a consistent gain of 17q sequences, and the specific gain of chromosome 17 following continuous in vitro culture of human ESCs is also common (see below”
Genomic alterations in cultured human embryonic stem cells (2005, background): “One group reported recurrent gains of chromosomes 17q and 12 (ref”
Human embryonic stem cells express an immunogenic nonhuman sialic acid (2005, background): “But feeder-free conditions seem to facilitate in vitro evolution of HESC, selecting for aneuploid cell”
Adaptation to culture of human embryonic stem cells and oncogenesis in vivo (2007, neutral): “H1a 53 47,XY,+12[3]/47,XY,+17[3]/48,XY,+12,+17[14]”
Dynamic Changes in the Copy Number of Pluripotency and Cell Proliferation Genes in Human ES and iPS Cells during Reprogramming and Time in Culture (2011, positive): “Human embryonic stem cell (hESC) lines have been shown to become aneuploid in culture (Baker et al., 2007; Draper et al., 2004; Imreh et al., 2006; Maitra et al., 2005; Mitalipova et al., 2005), and the most frequent changes, trisomies of chromosomes 12 and 17, are also characteristic of malignant…”