Feeder-independent culture of human embryonic stem cells

By T. Ludwig, Veit Bergendahl, M. Levenstein, Junying Yu, Mitchell D. Probasco, James A. Thomson. Nature Methods, 2006

712 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b8fa015766b5a4a3646f9f4c51b0c1ee79fc30d3

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Citations in the corpus

• Facilitators and Impediments of the Pluripotency Reprogramming Factors’ Initial Engagement with the Genome (2012, neutral): “For ChIP of the OSKM factors in ES/iPS cells, the chromatin was obtained from hFib-iPS cells at passage 15 and human H1-ES cells (Thomson et al., 1998) at passage 35 that were expanded under feeder-free conditions for three passages (Ludwig et al., 2006).”
• Production of De Novo Cardiomyocytes: Human Pluripotent Stem Cell Differentiation and Direct Reprogramming (2012, neutral): “Despite some difficulties in implementation (Akopian et al., 2010), several formulas have achieved widespread use, including XVIVO10 supplemented with FGF2 and TGF-b1 (Li et al., 2005) and the commercially available mTeSR1 (Ludwig et al., 2006) and StemPro hESC (Wang et al., 2007).”
• Whole-genome analysis of histone H3 lysine 4 and lysine 27 methylation in human embryonic stem cells. (2007, background): “300 Cell Stem Cell 1, 299–312, September 2007 a2007 Elsevie matin immunoprecipitation, ChIP) from H1 human ES cells maintained in defined medium (TeSR medium), (Figure 1A and see Figure S1A in the Supplemental Data available with this article online) (Ludwig et al., 2006a; Ludwig et al., 2006b).”
• Master Transcription Factors Determine Cell-Type-Specific Responses to TGF-β Signaling (2011, neutral): “Cell Culture hESCs (BGO3) were maintained as previously described (Ludwig et al., 2006) using mTESR1 media (Stem Cell Technologies), which contains TGF-b.”
• Chemically defined conditions for human iPS cell derivation and culture (2011, background): “Human ESCs (H1 and H9) were usually maintained in specific media on Matrigel-coated tissue culture plate”
• Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs (2010, background): “S9B shows that four naïve hESC lines and two naïve hiPSC lines cluster together and are different from a large number of hESCs and hiPSCs, including hESCs grown in mTESR1-defined culture conditions and Nanog-overexpressing hESCs, which can grow in a feeder-independent manner (32, 33).”