In vitro differentiation of transplantable neural precursors from human embryonic stem cells

By Su-Chun Zhang, Marius Wernig, I. Duncan, O. Brüstle, James A. Thomson. Nature Biotechnology, 2001

1,943 citations recorded by the source corpus.

Subfields: Developmental Neuroscience, Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/6fdfbbdb6967f42dee18c0451c60437baddce87e

How researchers see this paper

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In Developmental Neuroscience

1. G. Keller
2. Kenneth R. Boheler
3. A. Wobus
4. Alysson Renato Muotri
5. P. Kirwan
6. Juergen A. Knoblich
7. Fred H. Gage
8. Frederick J. Livesey

In Molecular Biology

1. G. Keller
2. K. Hochedlinger
3. G. Daley
4. R. Jaenisch
5. Kenneth R. Boheler
6. A. Wobus
7. Hynek Wichterle
8. J. A. Porter

In Surgery

1. G. Keller
2. Fred H. Gage

Citations in the corpus

• Human pluripotent stem cell-derived neural constructs for predicting neural toxicity (2015, background): “Human ES and iPS cells offer a consistent, scalable source for diverse neural cell types (49), including neural precursors that differentiate and self-assemble through mechanisms that recapitulate aspects of brain development (50–52).”
• Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency (2010, methodology): “FGF2 or Nogginwere added to cultures for thefirst 15 days, SB43152 was added from day 0–5 according to published protocols (26, 33).”
• Engineering Complex Tissues (2006, neutral): “A number of researchers are selecting specific cell types or groups of cells from EBs to apply to tissue repair.(22) While these proliferation and differentiation characteristics lead one to assume unlimited capacity for ESCs to make any tissue in the body, we are now just touching the surface on ho”
• Human embryonic stem cells express an immunogenic nonhuman sialic acid (2005, background): “Neural lineage cells derived from embryoid bodies express the polysialylated form of NCA”
• Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, background): “When transplanted into newborn mice, hES-cell-derived neural cells generated neurons and glia that could be detected in different regions of the recipient brain 4 wk later (Zhang et al. 2001).”
• Neural induction, the default model and embryonic stem cells (2002, neutral): “Similar to the mouse ES cells, most protocols for growth and culturing of human ES cells require FGF2 in the mediu”
• Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation (2005, background): “Although hESCs have been directed to differentiate into multipotent neural precursors (Zhang et al., 2001; Reubinoff et al., 2001; Carpenter et al., 2001), their differentiation to a purified neural population has thus far not been demonstrated.”
• Organogenesis in a dish: Modeling development and disease using organoid technologies (2014, background): “Brain Embryonic chick brain cells (45) Neural rosettes from mouse and human PSCs (48, 49) Cerebral organoids from mouse and human PSCs (28, 55) Pituitary Chick anterior pituitary (115) None Adenohypophysis organoids from mouse PSCs (73) Mammary gland Mammary gland from adult virgin mice (75) None 3D”
• A tumorigenic subpopulation with stem cell properties in melanomas. (2005, background): “medium contains bFGF, which has been used to induce neural differentiation of hESCs and bone marrow–derived stem cells (36, 42, 43).”
• Directed differentiation of embryonic stem cells into motor neurons. (2002, background): “In this study, we embryonic stem (ES) cells (Bain et al., 1995; Reubinoff have examined whether the signaling factors that operet al., 2001; Zhang et al., 2001; Rathjen et al., 2002).”