Directed differentiation of embryonic stem cells into motor neurons.

By Hynek Wichterle, I. Lieberam, J. A. Porter, T. Jessell. Cell, 2002

1,757 citations recorded by the source corpus.

Subfields: Developmental Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/bbff62017640879b5490b72cb35f63a2792cf75c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Developmental Neuroscience

1. G. Keller
2. Z. Kokaia
3. O. Lindvall
4. J. D. Macklis
5. Clifford J. Woolf
6. U. Shivraj Sohur
7. V. Tabar
8. B. Mitchell

In Molecular Biology

1. G. Keller
2. Hynek Wichterle
3. J. D. Macklis
4. V. Tabar
5. M. Maden
6. A. Schier
7. M. Carrasco
8. Diane D. A. Lu

Citations in the corpus

• Adult neurogenesis and cellular brain repair with neural progenitors, precursors and stem cells (2006, background): “…of neural precursor cells along specific cellular lineages during development and in the adult CNS will be critical to potential cellular therapeutic approaches for repopulating the damaged or diseased areas of the nervous system (Wichterle et al. 2002; Arlotta et al. 2005; Molyneaux et al. 2005).”
• Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, methodology): “Using the coculture approach together with the appropriate signaling molecules and selection steps, Wichterle et al. (2002) successfully generated cells that display many of the characteristics of motor neurons.”
• Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation (2005, methodology): “We induced the formation of neural-lineage cells by exposing cultures to the classic RA caudalizing protocol (Bain et al., 1995; Wichterle et al., 2002) with several modifications.”
• Non–cell autonomous effect of glia on motor neurons in an embryonic stem cell–based ALS model (2007, background): “Transgenic ESCs were differentiated into motor neurons in large number”
• Stem Cell Organoid Engineering (2016, neutral): “, 2005), and spinal cord motor neurons (Dimos et al., 2008; Li et al., 2005; Soundararajan et al., 2006; Wichterle et al., 2002) (see Petros et al.”
• Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons (2007, background): “12 ), and from mouse embryonic stem cells, as ESMNs not only show many of the molecular markers and functional properties of spinal motor neuron”
• REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis. (2005, background): “The differentiation occurs by pathways that recapitulate neuron generation in vivo ( Wichterle et al., 2002 ).”
• Specification of motoneurons from human embryonic stem cells (2005, background): “, with subsequent differentiation into large projection neurons, such as midbrain dopaminergic neurons and spinal motoneurons, in response to specific sets of morphogen”
• Opposing FGF and retinoid pathways control ventral neural pattern, neuronal differentiation, and segmentation during body axis extension. (2003, background): “We demonstrate that RA synthesis and et al., 1998), and more recently this has been shown toan intact RA pathway are required for somites to probe biased toward MN identity by further treatment withmote neuronal differentiation in vitro, and that in vivo Shh (Wichterle et al., 2002).”
• Stem cells for the treatment of neurological disorders (2006, background): “Recent reports have shown that it is possible to generate lower motor neurons in vitro from stem cells of various sources, including ES cells and those from the fetal CN”
• Conversion of Mouse and Human Fibroblasts into Functional Spinal Motor Neurons (2011, methodology): “First, as a positive control for this experiment, we generated iPSCs from the fibroblasts, then used retinoic acid and sonic hedgehog (Wichterle et al., 2002) to differentiate the iPSCs into motor neurons.”
• Microglia induce motor neuron death via the classical NF-κB pathway in amyotrophic lateral sclerosis (2014, positive): “We utilized an embryonic stem cell line containing an Hb9-GFP reporter, which we and others have shown recapitulate aspects of MN pathology and cell death when cocultured with ALS glia (Di Giorgio et al., 2007; Haidet-Phillips et al., 2011; Nagai et al., 2007; Wichterle et al., 2002).”